Jinshan Huang1,2, Na Liu1, Xingjia Shen1,2, Bifang Hao3,4. 1. Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, 212018, People's Republic of China. 2. Key Laboratory of Genetic Improvement of Sericulture in the Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu, 212018, People's Republic of China. 3. Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, 212018, People's Republic of China. bfhao@just.edu.cn. 4. Key Laboratory of Genetic Improvement of Sericulture in the Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu, 212018, People's Republic of China. bfhao@just.edu.cn.
Abstract
OBJECTIVES: To enhance the productivity of foreign protein in culture cells using baculovirus expression system. RESULTS: A low concentration of MβCD, with the optimal application concentration of 0.25 mM and the appropriate preincubation time range from 10 to 120 min, can efficiently enhance expression levels in both the AcMNPV and BmNPV expression systems. CONCLUSIONS: Preincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity.
OBJECTIVES: To enhance the productivity of foreign protein in culture cells using baculovirus expression system. RESULTS: A low concentration of MβCD, with the optimal application concentration of 0.25 mM and the appropriate preincubation time range from 10 to 120 min, can efficiently enhance expression levels in both the AcMNPV and BmNPV expression systems. CONCLUSIONS: Preincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity.