| Literature DB >> 31285614 |
Josephine Jüttner1,2, Arnold Szabo3, Brigitte Gross-Scherf1,2, Rei K Morikawa1,2, Santiago B Rompani1,2,4, Peter Hantz2,5, Tamas Szikra1,2, Federico Esposti2,6, Cameron S Cowan1,2, Arjun Bharioke1,2, Claudia P Patino-Alvarez1,2, Özkan Keles1,2, Akos Kusnyerik1,7, Thierry Azoulay8, Dominik Hartl2, Arnaud R Krebs2,9, Dirk Schübeler2,10, Rozina I Hajdu7, Akos Lukats3, Janos Nemeth7, Zoltan Z Nagy7, Kun-Chao Wu11, Rong-Han Wu11, Lue Xiang11, Xiao-Long Fang11, Zi-Bing Jin11, David Goldblum12, Pascal W Hasler12, Hendrik P N Scholl1,12,13, Jacek Krol14,15, Botond Roska16,17,18.
Abstract
Targeting genes to specific neuronal or glial cell types is valuable for both understanding and repairing brain circuits. Adeno-associated viruses (AAVs) are frequently used for gene delivery, but targeting expression to specific cell types is an unsolved problem. We created a library of 230 AAVs, each with a different synthetic promoter designed using four independent strategies. We show that a number of these AAVs specifically target expression to neuronal and glial cell types in the mouse and non-human primate retina in vivo and in the human retina in vitro. We demonstrate applications for recording and stimulation, as well as the intersectional and combinatorial labeling of cell types. These resources and approaches allow economic, fast and efficient cell-type targeting in a variety of species, both for fundamental science and for gene therapy.Entities:
Mesh：
Year: 2019 PMID： 31285614 DOI： 10.1038/s41593-019-0431-2
Source DB: PubMed Journal: Nat Neurosci ISSN： 1097-6256 Impact factor: 24.884