Literature DB >> 31282895

HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis.

Maria Teresa Gentile1, Olga Pastorino1, Maurizio Bifulco2, Luca Colucci-D'Amato3.   

Abstract

Angiogenesis is a phenomenon that includes different processes, such as endothelial cell proliferation, differentiation, and migration, that lead to the formation of new blood vessels and involve several signal transduction pathways. Here we show that the tube formation assay is a simple in vitro method to evaluate the impact of natural products on angiogenesis and to investigate the molecular mechanisms involved. In particular, in the presence of the water extract of Ruta graveolens (RGWE), endothelial cells are no longer able to form a cell-cell network and that the RGWE effects on human umbilical vein endothelial cell (HUVEC) tube formation is abolished by the constitutive activation of MEK.

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Year:  2019        PMID： 31282895     DOI： 10.3791/58591

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  7 in total

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Review 6.  Angiogenesis and Multiple Sclerosis Pathogenesis: A Glance at New Pharmaceutical Approaches.

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  7 in total

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