| Literature DB >> 31282211 |
Saharut Wongkaewkhiaw1,2, Suwimol Taweechaisupapong2,3, Chitchanok Anutrakunchai3, Kamran Nazmi4, Jan G M Bolscher4, Surasakdi Wongratanacheewin1,2, Sakawrat Kanthawong1,2,3.
Abstract
Melioidosis is a severe disease caused by Burkholderia pseudomallei. The biofilm of B. pseudomallei acquires resistance to several antibiotics and may be related to relapse in melioidosis patients. Here, the killing activity of antimicrobial peptides (LL-37, LL-31) and the D-enantiomers (D-LL-37, D-LL-31) in combination with ceftazidime (CAZ) against B. pseudomallei 1026b, H777 and a biofilm mutant M10, derived from H777 grown under biofilm-stimulating conditions was observed. Using static conditions, D-LL-31 exhibited the strongest killing activity against the three isolates in a dose-dependent manner. IC50 values for D-LL-31 ranged from 1 to 6 µM, for isolates M10, H777, and 1026b, respectively. Moreover, D-LL-31 combined with CAZ synergistically decreased the IC50 values of the peptide and antibiotic and caused also disruption of biofilms of B. pseudomallei 1026b under flow conditions. Thus a combination of D-LL-31 and CAZ may enhance the efficacy of the currently used antibiotic treatments against B. pseudomallei.Entities:
Keywords: Antimicrobial activity; D-enantiomer peptide; bacterial biofilm; cathelicidin peptides; melioidosis
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Year: 2019 PMID： 31282211 DOI： 10.1080/08927014.2019.1632835
Source DB: PubMed Journal: Biofouling ISSN： 0892-7014 Impact factor: 3.209