Jing Zhao1,2, Dan Liu1,2, Yuanyuan Wang1,3, Xiaofeng Zhu1,2, Yuanhu Xuan1,2, Xiaoyu Liu1,4, Haiyan Fan1,2, Lijie Chen1,2, Yuxi Duan1,2. 1. Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang, China. 2. College of Plant Protection, Shenyang Agricultural University, Shenyang, China. 3. College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China. 4. College of Sciences, Shenyang Agricultural University, Shenyang, China.
Abstract
BACKGROUND: The soybean cyst nematode Heterodera glycines (Ichinohe) is the most devastating pathogen affecting soybean production worldwide. Biocontrol agents have become eco-friendly candidates to control pathogens. The aim of this study was to discover novel biocontrol agents against H. glycines. RESULTS: Microbacterium maritypicum Sneb159, screened from 804 strains, effectively reduced the number of females in field experiments conducted in 2014 and 2015. The stability and efficiency of H. glycines control by Sneb159 was further assessed in growth chamber and field experiments. Sneb159 decreased H. glycines population densities, especially the number of females by 43.9%-67.7%. To confirm Sneb159 induced plant resistance, a split-root assay was conducted. Sneb159 induced local and systemic resistance to suppress the penetration and development of H. glycines, and enhanced the gene expression of PR2, PR3b, and JAZ1, involved in the salicylic acid and jasmonic acid pathways. CONCLUSION: This is the first report of M. maritypicum Sneb159 suppressing H. glycines infection. This effect may be the result of Sneb159-induced resistance. Our study indicates that M. maritypicum Sneb159 is a promising biocontrol agent against H. glycines.
BACKGROUND: The soybean cyst nematodeHeterodera glycines (Ichinohe) is the most devastating pathogen affecting soybean production worldwide. Biocontrol agents have become eco-friendly candidates to control pathogens. The aim of this study was to discover novel biocontrol agents against H. glycines. RESULTS:Microbacterium maritypicum Sneb159, screened from 804 strains, effectively reduced the number of females in field experiments conducted in 2014 and 2015. The stability and efficiency of H. glycines control by Sneb159 was further assessed in growth chamber and field experiments. Sneb159 decreased H. glycines population densities, especially the number of females by 43.9%-67.7%. To confirm Sneb159 induced plant resistance, a split-root assay was conducted. Sneb159 induced local and systemic resistance to suppress the penetration and development of H. glycines, and enhanced the gene expression of PR2, PR3b, and JAZ1, involved in the salicylic acid and jasmonic acid pathways. CONCLUSION: This is the first report of M. maritypicum Sneb159 suppressing H. glycinesinfection. This effect may be the result of Sneb159-induced resistance. Our study indicates that M. maritypicum Sneb159 is a promising biocontrol agent against H. glycines.
Authors: Felipe Martins do Rêgo Barros; Alexandre Pedrinho; Lucas William Mendes; Caio César Gomes Freitas; Fernando Dini Andreote Journal: Appl Environ Microbiol Date: 2022-08-24 Impact factor: 5.005