| Literature DB >> 31278897 |
Xiaonan Liu1, Chao Zhang1, Kai Hua2, Jianping Liang1, Hang Li1, Ting Ma3, Juanli Zhu4, Yali Cui5.
Abstract
In order to simplify biological sample preparation to meet the demand of rapid genotyping, we improve alkaline polyethylene glycol (APEG) based on Chomczynski's procedure for the PCR-based lateral flow genotyping system, which enables the rapid and efficient direct genotyping from whole blood without DNA purification. The improved APEG has a high tolerance for extreme storage conditions. Testing whole blood with an abnormal hematological index indicates that APEG efficiency would not be influenced by pathological factors. Compared with sequencing, the accuracy of this genotyping system was 100% on testing with 200 clinical samples.Entities:
Keywords: Alkaline polyethylene glycol; Direct genotyping; Whole blood
Year: 2019 PMID： 31278897 DOI： 10.1016/j.ab.2019.113351
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365