S N Slavov1, R Maraninchi Silveira2, M R Hespanhol2, V Sauvage3, E S Rodrigues2, L Fontanari Krause4, H T Bittencourt5, V Caro6, S Laperche3, D T Covas2, S Kashima2. 1. Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil; Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil. Electronic address: svetoslav.slavov@hemocentro.fmrp.usp.br. 2. Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14051-140 Ribeirão Preto, São Paulo, Brazil. 3. Institut national de la transfusion Sanguine (INTS), département d'études des Agents transmissibles par le sang (DATS), Centre national de référence risques infectieux transfusionnels, 75015 Paris, France. 4. Laboratory of Biosciences, Franciscan University, 97010-030 Santa Maria, Rio Grande do Sul, Brazil. 5. Institute of Hematology and Hemotherapy of Amapá, 68900-074 Macapá, Amapá, Brazil. 6. Pole for Genotyping of Pathogens (PGP), Laboratory for Urgent Response to Biological Threats, Environment and Infectious Risks Research and Expertise Unit, Institut Pasteur, 75724 Paris, France.
Abstract
OBJECTIVES: The objectives of this study were to evaluate the prevalence of Human Pegivirus-1 (HPgV-1) viremia and genotype diversity among healthy blood donors from the Eastern Brazilian Amazon (city of Macapá, State of Amapá). There is little information for prevalence and circulation of HPgV-1 in this remote Brazilian region. MATERIALS AND METHODS: We conducted a study evaluating the HPgV-1 RNA prevalence and circulating genotypes in 431 volunteer blood donors originating from the Eastern Brazilian Amazon. The obtained HPgV-1 positive samples were submitted to sequencing and genotyping analysis in order to examine the genotype diversity of this virus in the Brazilian Amazon. RESULTS: Our results demonstrated a prevalence of HPgV-1 RNA in 9.5% of the tested blood donors. The phylogenetic analyses of the detected positive samples showed the presence of HPgV-1 genotypes 1, 2 and 3. The most frequently detected genotype was 2 (78.0% of the cases) represented by sub-genotypes 2A (39.0%) and 2B (39.0%). At lower rates, genotypes 1 (14.6%) and 3 (7.4%) were also detected. CONCLUSION: Our results revealed the presence of genotypes with European, Asiatic and African endemicity in Amazonian blood donors, probably due to the complex miscegenation processes that took place in this Brazilian region. More investigations, including information for the prevalence of HPgV-1 RNA in blood donors from other Latin American countries are needed to estimate the viremic rates and genotype distribution of this virus in a highly diverse continent like South America.
OBJECTIVES: The objectives of this study were to evaluate the prevalence of Human Pegivirus-1 (HPgV-1) viremia and genotype diversity among healthy blood donors from the Eastern Brazilian Amazon (city of Macapá, State of Amapá). There is little information for prevalence and circulation of HPgV-1 in this remote Brazilian region. MATERIALS AND METHODS: We conducted a study evaluating the HPgV-1 RNA prevalence and circulating genotypes in 431 volunteer blood donors originating from the Eastern Brazilian Amazon. The obtained HPgV-1 positive samples were submitted to sequencing and genotyping analysis in order to examine the genotype diversity of this virus in the Brazilian Amazon. RESULTS: Our results demonstrated a prevalence of HPgV-1 RNA in 9.5% of the tested blood donors. The phylogenetic analyses of the detected positive samples showed the presence of HPgV-1 genotypes 1, 2 and 3. The most frequently detected genotype was 2 (78.0% of the cases) represented by sub-genotypes 2A (39.0%) and 2B (39.0%). At lower rates, genotypes 1 (14.6%) and 3 (7.4%) were also detected. CONCLUSION: Our results revealed the presence of genotypes with European, Asiatic and African endemicity in Amazonian blood donors, probably due to the complex miscegenation processes that took place in this Brazilian region. More investigations, including information for the prevalence of HPgV-1 RNA in blood donors from other Latin American countries are needed to estimate the viremic rates and genotype distribution of this virus in a highly diverse continent like South America.
Authors: Marcus Vinícius Guimarães Lacerda; Victor Hugo Aquino; Mario Luis Garcia de Figueiredo; Evan P Williams; Colleen B Jonsson; Mohd Jaseem Khan; Márcio Roberto Teixeira Nunes; Clayton Pereira Silva de Lima; Luiz Tadeu Moraes Figueiredo; Mônica Regina Farias Costa; Maria Paula Gomes Mourão Journal: Arch Virol Date: 2022-07-16 Impact factor: 2.685
Authors: M A L Doan; A Roczkowsky; M Smith; G Blevins; F K H van Landeghem; B B Gelman; W G Branton; J T Stapleton; T C Hobman; C Power Journal: J Virol Date: 2021-09-15 Impact factor: 5.103
Authors: Aniel de Sarom Negrão Silva; Clayton Pereira Silva; Rafael Ribeiro Barata; Pedro Victor Reis da Silva; Patrícia Danin Jordão Monteiro; Letícia Lamarão; Rommel Mário Rodríguez Burbano; Márcio Roberto Teixeira Nunes; Patrícia Danielle Lima de Lima Journal: Virol J Date: 2020-10-14 Impact factor: 4.099