Sara Sangi1, Maria L C Santos1, Camilla R Alexandrino2, Maura Da Cunha2, Fernanda S Coelho1, Gabrielly P Ribeiro3, Dominik Lenz3, Helkin Ballesteros4, Adriana S Hemerly4, Thiago M Venâncio1, Antônia E A Oliveira1, Clícia Grativol5. 1. Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, P5, 228, Parque Califórnia, Campos dos Goytacazes, Rio de Janeiro, Brazil. 2. Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Brazil. 3. Departmento de Ciências Farmacêuticas, Universidade de Vila Velha, Vila Velha, Brazil. 4. Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica Leopoldo De Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. 5. Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, P5, 228, Parque Califórnia, Campos dos Goytacazes, Rio de Janeiro, Brazil. cgrativol@uenf.br.
Abstract
MAIN CONCLUSION: Identification of the structural changes and cell wall-related genes likely involved in cell wall extension, cellular water balance and cell wall biosynthesis on embryonic axes during germination of soybean seeds. Cell wall is a highly organized and dynamic structure that provides mechanical support for the cell. During seed germination, the cell wall is critical for cell growth and seedling establishment. Although seed germination has been widely studied in several species, key aspects regarding the regulation of cell wall dynamics in germinating embryonic axes remain obscure. Here, we characterize the gene expression patterns of cell wall pathways and investigate their impact on the cell wall dynamics of embryonic axes of germinating soybean seeds. We found 2143 genes involved in cell wall biosynthesis and assembly in the soybean genome. Key cell wall genes were highly expressed at specific germination stages, such as expansins, UDP-Glc epimerases, GT family, cellulose synthases, peroxidases, arabinogalactans, and xyloglucans-related genes. Further, we found that embryonic axes grow through modulation of these specific cell wall genes with no increment in biomass. Cell wall structural analysis revealed a defined pattern of cell expansion and an increase in cellulose content during germination. In addition, we found a clear correlation between these structural changes and expression patterns of cell wall genes during germination. Taken together, our results provide a better understanding of the complex transcriptional regulation of cell wall genes that drive embryonic axes growth and expansion during soybean germination.
MAIN CONCLUSION: Identification of the structural changes and cell wall-related genes likely involved in cell wall extension, cellular water balance and cell wall biosynthesis on embryonic axes during germination of soybean seeds. Cell wall is a highly organized and dynamic structure that provides mechanical support for the cell. During seed germination, the cell wall is critical for cell growth and seedling establishment. Although seed germination has been widely studied in several species, key aspects regarding the regulation of cell wall dynamics in germinating embryonic axes remain obscure. Here, we characterize the gene expression patterns of cell wall pathways and investigate their impact on the cell wall dynamics of embryonic axes of germinating soybean seeds. We found 2143 genes involved in cell wall biosynthesis and assembly in the soybean genome. Key cell wall genes were highly expressed at specific germination stages, such as expansins, UDP-Glc epimerases, GT family, cellulose synthases, peroxidases, arabinogalactans, and xyloglucans-related genes. Further, we found that embryonic axes grow through modulation of these specific cell wall genes with no increment in biomass. Cell wall structural analysis revealed a defined pattern of cell expansion and an increase in cellulose content during germination. In addition, we found a clear correlation between these structural changes and expression patterns of cell wall genes during germination. Taken together, our results provide a better understanding of the complex transcriptional regulation of cell wall genes that drive embryonic axes growth and expansion during soybean germination.
Authors: I Amaya; M A Botella; M de la Calle; M I Medina; A Heredia; R A Bressan; P M Hasegawa; M A Quesada; V Valpuesta Journal: FEBS Lett Date: 1999-08-20 Impact factor: 4.124
Authors: Bruce Downie; Sunitha Gurusinghe; Petambar Dahal; Richard R Thacker; John C Snyder; Hiroyuki Nonogaki; Kyuock Yim; Keith Fukanaga; Veria Alvarado; Kent J Bradford Journal: Plant Physiol Date: 2003-03 Impact factor: 8.340
Authors: Sara Sangi; Paula M Araújo; Fernanda S Coelho; Rajesh K Gazara; Fabrício Almeida-Silva; Thiago M Venancio; Clicia Grativol Journal: Plants (Basel) Date: 2021-01-16