Kimberly E Martin1, Paul B Pencharz1,2,3, Mahroukh Rafii1, Ronald O Ball2,4, Sylwia Szwiega1, Rajavel Elango5,6, Glenda Courtney-Martin1,2,7. 1. Research Institute, Hospital for Sick Children, Toronto, Canada. 2. Department of Nutritional Sciences, University of Toronto, Toronto, Canada. 3. Department of Pediatrics, University of Toronto, Toronto, Canada. 4. Department of Agriculture, Food, and Nutritional Science, University of Alberta, Edmonton, Canada. 5. Department of Pediatrics, School of Population and Public Health, University of British Columbia, Vancouver, Canada. 6. BC Children's Hospital Research Institute, BC Children's Hospital, Vancouver, Canada. 7. Faculty of Kinesiology and Physical Education, University of Toronto, Toronto, Canada.
Abstract
BACKGROUND: The phenylalanine requirement of the elderly is not known. Current recommendations are based on studies in young adults and are derived from a combined estimate of the total aromatic amino acids, phenylalanine, and tyrosine. OBJECTIVES: The purpose of this study was to determine the dietary phenylalanine requirement of adults aged >65 y, using the direct amino acid oxidation method, by measuring the oxidation of l-[1-13C]phenylalanine to 13CO2 in response to graded phenylalanine intakes in the presence of excess tyrosine. METHODS:Twelve subjects (6 men, 6 women), aged 73.8 ±6.7 y (mean ± SD) and with a BMI (in kg/m2) of 26.4 ± 4.8 and 25.2 ± 4.4 for men and women, respectively, were randomized to phenylalanine intakes ranging from 7.20 to 40.0 mg .kg-1 .d-1 for a total of 66 studies. Study diets were isocaloric and isonitrogenous, providing protein and energy at 1.0 g .kg-1 .d-1 and 1.5 × resting energy expenditure (REE), respectively. Protein was provided as an amino acid mixture patterned after egg protein, with an excess of tyrosine and alanine to balance the nitrogen as phenylalanine intakes were varied. Two days prior to the study day, subjects were adapted to a milkshake diet providing protein at 1.0 g.kg-1 .d-1 and energy at 1.7 × REE. The mean phenylalanine requirement was determined using biphase linear regression analysis, which identified a breakpoint in the F13CO2 in response to graded phenylalanine intakes. RESULTS: The mean and upper 95% CIs (approximating the recommended dietary allowance) of phenylalanine requirements were estimated to be 9.03 and 15.9 mg.kg-1 .d-1, respectively. CONCLUSION: These results are similar to previously derived estimates of 9.1 and 13.6 mg.kg-1 .d-1 in young adult men and suggest that higher protein needs of the elderly to stimulate similar muscle protein synthesis rates as young adults are not driven by an increased requirement for phenylalanine. This trial was registered at clinicaltrials.gov as NCT02971059.
RCT Entities:
BACKGROUND: The phenylalanine requirement of the elderly is not known. Current recommendations are based on studies in young adults and are derived from a combined estimate of the total aromatic amino acids, phenylalanine, and tyrosine. OBJECTIVES: The purpose of this study was to determine the dietary phenylalanine requirement of adults aged >65 y, using the direct amino acid oxidation method, by measuring the oxidation of l-[1-13C]phenylalanine to 13CO2 in response to graded phenylalanine intakes in the presence of excess tyrosine. METHODS: Twelve subjects (6 men, 6 women), aged 73.8 ± 6.7 y (mean ± SD) and with a BMI (in kg/m2) of 26.4 ± 4.8 and 25.2 ± 4.4 for men and women, respectively, were randomized to phenylalanine intakes ranging from 7.20 to 40.0 mg .kg-1 .d-1 for a total of 66 studies. Study diets were isocaloric and isonitrogenous, providing protein and energy at 1.0 g .kg-1 .d-1 and 1.5 × resting energy expenditure (REE), respectively. Protein was provided as an amino acid mixture patterned after egg protein, with an excess of tyrosine and alanine to balance the nitrogen as phenylalanine intakes were varied. Two days prior to the study day, subjects were adapted to a milkshake diet providing protein at 1.0 g.kg-1 .d-1 and energy at 1.7 × REE. The mean phenylalanine requirement was determined using biphase linear regression analysis, which identified a breakpoint in the F13CO2 in response to graded phenylalanine intakes. RESULTS: The mean and upper 95% CIs (approximating the recommended dietary allowance) of phenylalanine requirements were estimated to be 9.03 and 15.9 mg.kg-1 .d-1, respectively. CONCLUSION: These results are similar to previously derived estimates of 9.1 and 13.6 mg.kg-1 .d-1 in young adult men and suggest that higher protein needs of the elderly to stimulate similar muscle protein synthesis rates as young adults are not driven by an increased requirement for phenylalanine. This trial was registered at clinicaltrials.gov as NCT02971059.
Authors: T Brock Symons; Scott E Schutzler; Tara L Cocke; David L Chinkes; Robert R Wolfe; Douglas Paddon-Jones Journal: Am J Clin Nutr Date: 2007-08 Impact factor: 7.045
Authors: Mahroukh Rafii; Karen Chapman; Jillian Owens; Rajavel Elango; Wayne W Campbell; Ronald O Ball; Paul B Pencharz; Glenda Courtney-Martin Journal: J Nutr Date: 2014-10-15 Impact factor: 4.798
Authors: Edgar L Dillon; Melinda Sheffield-Moore; Douglas Paddon-Jones; Charles Gilkison; Arthur P Sanford; Shanon L Casperson; Jie Jiang; David L Chinkes; Randall J Urban Journal: J Clin Endocrinol Metab Date: 2009-02-10 Impact factor: 5.958