Irina Y Nikitina1, Natalya L Karpina2, Olga V Kasimceva3, Vladislav Y Gergert4, Atadzhan Ergeshov5, Irina V Lyadova6. 1. Immunology Department, Central Tuberculosis Research Institute, Yauzskaya Alley, 2, Moscow 107564, Russia. Electronic address: redwings2009@yandex.ru. 2. Diagnostic Outpatient Department, Central Tuberculosis Research Institute, Yauzskaya Alley, 2, Moscow 107564, Russia. Electronic address: natalya-karpina@rambler.ru. 3. Diagnostic Outpatient Department, Central Tuberculosis Research Institute, Yauzskaya Alley, 2, Moscow 107564, Russia. Electronic address: kab__@mail.ru. 4. Immunology Department, Central Tuberculosis Research Institute, Yauzskaya Alley, 2, Moscow 107564, Russia. Electronic address: hergertv@mail.ru. 5. Administrative Department, Central Tuberculosis Research Institute, Yauzskaya Alley, 2, Moscow 107564, Russia. Electronic address: cniit@ctri.ru. 6. Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Vavilova Str., 26, Moscow 119334, Russia; Immunology Department, Central Tuberculosis Research Institute, Yauzskaya Alley, 2, Moscow 107564, Russia. Electronic address: ivlyadova@mail.ru.
Abstract
BACKGROUND: The early identification of Mycobacterium tuberculosis infection can prevent tuberculosis (TB) transmission. A skin test with a tuberculosis recombinant allergen (Diaskintest) is a new method for identification that has been implemented in Russia. This study was performed to compare the performances of Diaskintest and QuantiFERON-TB Gold (QFT) in adults and children with suspected TB in Moscow, Russia. METHODS: Adults (n=85) and children (n=96) were tested using Diaskintest and QFT. Concordance and comparative analyses were performed. RESULTS: Diaskintest and QFT were concordant in 84% of adults and 90% of children (overall concordance 87%, κ>0.6, Kc>0.5). The concordance between QFT, Diaskintest, and the final diagnosis was good in adults (86% and 81%, respectively) and moderate in children (77% and 79%, respectively). In adults, QFT had a higher sensitivity for detecting TB than Diaskintest (82% and 68%, respectively); in children, Diaskintest was more sensitive (73% and 65%, respectively). In patients with a confirmed TB diagnosis, negative Diaskintest/QFT results were associated with low disease activity. Combined Diaskintest/QFT results identified TB patients with higher sensitivity and specificity than each test separately. CONCLUSIONS: Diaskintest is a low-cost diagnostic tool that shows a test positivity rate similar to QFT and can be used in combination with QFT as an adjunctive test for TB diagnosis.
BACKGROUND: The early identification of Mycobacterium tuberculosis infection can prevent tuberculosis (TB) transmission. A skin test with a tuberculosis recombinant allergen (Diaskintest) is a new method for identification that has been implemented in Russia. This study was performed to compare the performances of Diaskintest and QuantiFERON-TB Gold (QFT) in adults and children with suspected TB in Moscow, Russia. METHODS: Adults (n=85) and children (n=96) were tested using Diaskintest and QFT. Concordance and comparative analyses were performed. RESULTS: Diaskintest and QFT were concordant in 84% of adults and 90% of children (overall concordance 87%, κ>0.6, Kc>0.5). The concordance between QFT, Diaskintest, and the final diagnosis was good in adults (86% and 81%, respectively) and moderate in children (77% and 79%, respectively). In adults, QFT had a higher sensitivity for detecting TB than Diaskintest (82% and 68%, respectively); in children, Diaskintest was more sensitive (73% and 65%, respectively). In patients with a confirmed TB diagnosis, negative Diaskintest/QFT results were associated with low disease activity. Combined Diaskintest/QFT results identified TB patients with higher sensitivity and specificity than each test separately. CONCLUSIONS: Diaskintest is a low-cost diagnostic tool that shows a test positivity rate similar to QFT and can be used in combination with QFT as an adjunctive test for TB diagnosis.
Authors: Giovanni Battista Migliori; Catherine W M Ong; Linda Petrone; Lia D'Ambrosio; Rosella Centis; Delia Goletti Journal: Breathe (Sheff) Date: 2021-09