Amit Khurana1, Pratibha Anchi1, Prince Allawadhi2, Vinay Kumar2, Nilofer Sayed1, Gopinath Packirisamy2,3, Chandraiah Godugu1. 1. Department of Regulatory Toxicology, National Institute of Pharmaceutical Education & Research (NIPER), Balanagar, Hyderabad, Telangana, India. 2. Department of Biotechnology, Indian Institute of Technology-Roorkee, Roorkee, Uttarakhand-247667, India. 3. Nanobiotechnology Laboratory, Centre for Nanotechnology, Indian Institute of Technology-Roorkee, Roorkee, Uttarakhand-247667, India.
Abstract
Aim: The present study was carried out to assess the effect of nanoceria (NC) on pancreatic inflammation caused by cerulein. Methods: NC was characterized and in vitro studies were carried out in murine macrophages. The in vivo effects were tested on cerulein-induced pancreatitis. Results: In vitro treatment with NC remarkably protected macrophages from lipopolysaccharide-induced inflammation and oxidative stress as evident from the results of 2',7'-dichlorofluorescin diacetate, JC-1 and MitoSox staining. In vivo treatment with NC showed potent superoxide dismutase and catalase mimetic activity, antipancreatitis activity and improved histology. Furthermore, it reduced the expression of p65-NF-κB and acetylation of histone H3 at lysine K14, K56 and K79 residues. Conclusion: We for the first time, demonstrate that NC may be a promising candidate for the therapy of pancreatitis.
Aim: The present study was carried out to assess the effect of nanoceria (NC) on pancreatic inflammation caused by cerulein. Methods:NC was characterized and in vitro studies were carried out in murine macrophages. The in vivo effects were tested on cerulein-induced pancreatitis. Results: In vitro treatment with NC remarkably protected macrophages from lipopolysaccharide-induced inflammation and oxidative stress as evident from the results of 2',7'-dichlorofluorescin diacetate, JC-1 and MitoSox staining. In vivo treatment with NC showed potent superoxide dismutase and catalase mimetic activity, antipancreatitis activity and improved histology. Furthermore, it reduced the expression of p65-NF-κB and acetylation of histone H3 at lysine K14, K56 and K79 residues. Conclusion: We for the first time, demonstrate that NC may be a promising candidate for the therapy of pancreatitis.
Authors: Elidamar Nunes de Carvalho Lima; Ana Luiza Moraes Octaviano; José Roberto Castilho Piqueira; Ricardo Sobhie Diaz; João Francisco Justo Journal: Int J Nanomedicine Date: 2022-02-21