| Literature DB >> 31267461 |
Daniel Some1, Vladimir Razinkov2.
Abstract
We present a review of high-throughput techniques for the characterization and quality control of proteins in the course of purification, evaluation, and formulation, based on static and dynamic light scattering. Multi-angle static light scattering (MALS) in combination with rapid, low-volume UHPLC size exclusion chromatography is effective in characterizing key biophysical properties, while dynamic light scattering (DLS) in high-throughput microwell-plate format provides large quantities of data in a short time to screen many conditions, excipients, cell lines, or candidate biotherapeutics.Keywords: Aggregation; Dynamic light scattering; Monoclonal antibodies; Protein formulation; Protein quality; SEC-MALS
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Year: 2019 PMID： 31267461 DOI： 10.1007/978-1-4939-9624-7_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745