Pablo Iruzubieta1,2, Marta Monzón3, Tomás Castiella4,5, Teresa Ramírez4, Concepción Junquera3,5. 1. Department of Human Anatomy and Histology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain. pablo.iruzubieta@hotmail.es. 2. Institute for Health Research Aragón (IIS), C/Domingo Miral s/n, 50009, Zaragoza, Spain. pablo.iruzubieta@hotmail.es. 3. Department of Human Anatomy and Histology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain. 4. Department of Pathological Anatomy, Legal and Forensic Medicine and Toxicology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain. 5. Institute for Health Research Aragón (IIS), C/Domingo Miral s/n, 50009, Zaragoza, Spain.
Abstract
BACKGROUND: Gastrointestinal stromal tumour (GIST) is a mesenchymal cancer which derives from interstitial cells of Cajal. To determine whether a relationship between Hedgehog (Hh) signalling pathway and primary cilia exists in GIST tumours is intended here. METHODS: Immunohistochemical, immunofluorescence and ultrastructural techniques were performed in this study. RESULTS: We show that GIST cells present primary cilia (an antenna-like structure based on microtubules). But, moreover, we prove Hedgehog signalling pathway activation in these tumours (a pathway related with tumoural features such as proliferation, migration or stemness) and we show for the first time that this signalling pathway activation in GIST is mediated by primary cilia, likely in a paracrine way. CONCLUSION: Thus, primary cilia and Hedgehog signalling would be fundamental in tumoural microenvironment control of GIST cells for their maintenance, differentiation and proliferation.
BACKGROUND:Gastrointestinal stromal tumour (GIST) is a mesenchymal cancer which derives from interstitial cells of Cajal. To determine whether a relationship between Hedgehog (Hh) signalling pathway and primary cilia exists in GIST tumours is intended here. METHODS: Immunohistochemical, immunofluorescence and ultrastructural techniques were performed in this study. RESULTS: We show that GIST cells present primary cilia (an antenna-like structure based on microtubules). But, moreover, we prove Hedgehog signalling pathway activation in these tumours (a pathway related with tumoural features such as proliferation, migration or stemness) and we show for the first time that this signalling pathway activation in GIST is mediated by primary cilia, likely in a paracrine way. CONCLUSION: Thus, primary cilia and Hedgehog signalling would be fundamental in tumoural microenvironment control of GIST cells for their maintenance, differentiation and proliferation.
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