| Literature DB >> 3126485 |
R Pine1, D E Levy, N Reich, J E Darnell.
Abstract
Genes in human chromosomes that normally require induction by alpha-interferon are activated after calcium phosphate (CaPO4) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by gamma-interferon also are affected by CaPO4-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.Entities:
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Year: 1988 PMID: 3126485 PMCID: PMC336321 DOI: 10.1093/nar/16.4.1371
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971