Literature DB >> 3126242

A sensitive method of detecting proteins on dot and Western blots using a monoclonal antibody to FITC.

D Samuel1, R J Patt, R A Abuknesha.   

Abstract

Monoclonal antibodies to FITC were produced and shown to be specific for the fluorochrome. Molecular weight marker proteins labelled with FITC could be detected after SDS-PAGE and transfer onto nitrocellulose using anti-FITC followed by an anti-mouse IgG-alkaline phosphatase conjugate. The molecular weight of an antigen common to Legionella pneumophila and recognised by a monoclonal antibody could be determined accurately on a Western blot when FITC labelled markers were used as internal standards. The FITC-anti-FITC system was shown to be extremely sensitive, detecting 23.7 amol of BSA-FITC conjugate (equivalent to 1.42 x 10(7) molecules of FITC) in a dot blot assay.

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Year:  1988        PMID： 3126242     DOI： 10.1016/0022-1759(88)90221-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  9 in total

1.  Rapid immunofluorescence microscopy for diagnosis of melioidosis.

Authors:  Vanaporn Wuthiekanun; Varunee Desakorn; Gumphol Wongsuvan; Premjit Amornchai; Allen C Cheng; Bina Maharjan; Direk Limmathurotsakul; Wirongrong Chierakul; Nicholas J White; Nicholas P J Day; Sharon J Peacock
Journal:  Clin Diagn Lab Immunol       Date:  2005-04

2.  Reduction of the rate of fluorescence decay of FITC- and carboxyfluorescein-stained cells by anti-FITC antibodies.

Authors:  R A Abuknesha; H M al-Mazeedi; R G Price
Journal:  Histochem J       Date:  1992-02

3.  Evaluation of urinary antigen ELISA for diagnosing Legionella pneumophila serogroup 1 infection.

Authors:  R J Birtles; T G Harrison; D Samuel; A G Taylor
Journal:  J Clin Pathol       Date:  1990-08       Impact factor: 3.411

4.  Immunofluorescence microscopy for the rapid diagnosis of melioidosis.

Authors:  A L Walsh; M D Smith; V Wuthiekanun; Y Suputtamongkol; V Desakorn; W Chaowagul; N J White
Journal:  J Clin Pathol       Date:  1994-04       Impact factor: 3.411

5.  AMC-anti-FITC conjugates: novel reagents for amplified immunochemical techniques. Immunofluorescent staining of human fibroblasts.

Authors:  R A Abuknesha; H M al-Mazeedi; R G Price
Journal:  Histochem J       Date:  1992-09

6.  Monoclonal antibodies show Listeria monocytogenes in necropsy tissue samples.

Authors:  J McLauchlin; A Black; H T Green; J Q Nash; A G Taylor
Journal:  J Clin Pathol       Date:  1988-09       Impact factor: 3.411

7.  A high-throughput, precipitating colorimetric sandwich ELISA microarray for Shiga toxins.

Authors:  Andrew Gehring; Xiaohua He; Pina Fratamico; Joseph Lee; Lori Bagi; Jeffrey Brewster; George Paoli; Yiping He; Yanping Xie; Craig Skinner; Charlie Barnett; Douglas Harris
Journal:  Toxins (Basel)       Date:  2014-06-11       Impact factor: 4.546

8.  Detection of rubella virus-specific immunoglobulin G in saliva by an amplification-based enzyme-linked immunosorbent assay using monoclonal antibody to fluorescein isothiocyanate.

Authors:  A J Vyse; D W Brown; B J Cohen; R Samuel; D J Nokes
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

9.  Diagnosis of Penicillium marneffei infection by quantitation of urinary antigen by using an enzyme immunoassay.

Authors:  V Desakorn; M D Smith; A L Walsh; A J Simpson; D Sahassananda; A Rajanuwong; V Wuthiekanun; P Howe; B J Angus; P Suntharasamai; N J White
Journal:  J Clin Microbiol       Date:  1999-01       Impact factor: 5.948
  9 in total

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