Sarah F Hamm-Alvarez1,2, Curtis T Okamoto2, Srikanth R Janga1, Danielle Feigenbaum3, Maria C Edman1, Daniel Freire3, Mihir Shah1, Raveena Ghanshani1,3, Wendy J Mack4, Mark F Lew3. 1. Department of Ophthalmology & Roski Eye Institute, Keck School of Medicine, Los Angeles, CA 90033-6103, USA. 2. Department of Pharmacology & Pharmaceutical Sciences, School of Pharmacy, Los Angeles, CA 90033-6103, USA. 3. Department of Neurology, Keck School of Medicine, Los Angeles, CA 90033-6103, USA. 4. Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-6103, USA.
Abstract
Aim: Secretion of proteins into basal tears of Parkinson's disease (PD) patients may be altered by changes in nerve function. Materials & methods: Oligomeric α-SynOligo and total α-SynTotal, CCL-2, DJ-1, LF and MMP-9 were measured in basal tears from 93 PD patients and 82 age- and sex-equivalent healthy controls. Results: α-SynTotal was decreased (p = 0.0043), whereas α-SynOligo (p < 0.0001) and the ratio of α-SynOligo/α-SynTotal (p < 0.0001) were increased in basal tears from PD patients compared with healthy controls. Area under receiver-operating curves of α-SynOligo and α-SynOligo/α-SynTotal contents were 0.70 (95% confidence limits: 0.621-0.774) and 0.72 (95% confidence limits: 0.642-0.792). Conclusion: PD patient basal tears may contain biomarkers that can be assayed noninvasively and inexpensively.
Aim: Secretion of proteins into basal tears of Parkinson's disease (PD) patients may be altered by changes in nerve function. Materials & methods: Oligomeric α-SynOligo and total α-SynTotal, CCL-2, DJ-1, LF and MMP-9 were measured in basal tears from 93 PDpatients and 82 age- and sex-equivalent healthy controls. Results: α-SynTotal was decreased (p = 0.0043), whereas α-SynOligo (p < 0.0001) and the ratio of α-SynOligo/α-SynTotal (p < 0.0001) were increased in basal tears from PDpatients compared with healthy controls. Area under receiver-operating curves of α-SynOligo and α-SynOligo/α-SynTotal contents were 0.70 (95% confidence limits: 0.621-0.774) and 0.72 (95% confidence limits: 0.642-0.792). Conclusion:PDpatient basal tears may contain biomarkers that can be assayed noninvasively and inexpensively.
Authors: Marlies Gijs; Inez H G B Ramakers; Pieter Jelle Visser; Frans R J Verhey; Marjo P H van de Waarenburg; Casper G Schalkwijk; Rudy M M A Nuijts; Carroll A B Webers Journal: Sci Rep Date: 2021-11-22 Impact factor: 4.379
Authors: Shruti Singh Kakan; Maria C Edman; Alexander Yao; Curtis T Okamoto; Annie Nguyen; Brooke E Hjelm; Sarah F Hamm-Alvarez Journal: Front Immunol Date: 2022-03-04 Impact factor: 7.561