Modification of intracellular glucose metabolism in human skin fibroblast preincubated with p-chlorophenoxyisobutyrate.

1. The effect of p-chlorophenoxyisobutyrate (CPIB) on glucose metabolism human skin fibroblasts was examined. 2. CPIB increased the incorporation of 2-deoxy-D-[U-14C]glucose into skin fibroblasts. 3. CPIB decreased [14C]CO2 production from D-[U-14C]glucose but did not affect pyruvate dehydrogenase activity. 4. CPIB reduced fatty acid oxidation activity and cholesterol synthesis but increased triglyceride synthesis. 5. These effects of CPIB were observed both in the presence and in the absence of insulin. 6. One possible mechanism of CPIB on reducing plasma glucose may be due to the increase of glucose incorporation into the cells and triglyceride synthesis in the cells.