Modulation of acetylcholine release from guinea-pig Ileum myenteric plexus by arachidonic acid cascade inhibitors.

Effects of arachidonic acid and mepacrine on ACh release from guinea-pig ileum myenteric plexus were investigated. Mepacrine (1-8 microM) inhibited the ACh release in a concentration-dependent manner. Arachidonic acid counteracted the inhibitory effect of mepacrine, but PGE2 did not. The inhibition induced by a combination of mepacrine and indomethacin on nicotine-induced ACh release was prevented by arachidonic acid, while that on spontaneous ACh release was prevented by arachidonic acid and PGE2 added simultaneously. The roles of arachidonic acid and PGs in the ACh release will be discussed.