| Literature DB >> 31253343 |
Abdelrahim Hussein Abdelazeem Hassan1, José Francisco Bergua2, Eden Morales-Narváez3, Arben Mekoçi4.
Abstract
Considering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on non-radiative energy transfer between graphene oxide and quantum dots for determination of E. coli O157:H7 in beef and river water. FLFIA showed a high sensitivity rate thousand-fold better than the conventional lateral flow (LF). In inoculated minced beef and river water samples, the limits of detection were 178 and 133 CFU g-1 or mL-1, respectively. Besides, it presented a high selectivity in the presence of other possible interfering bacteria. The single antibody approach reduced the assay cost to 60% less than the conventional LF. Alongside, the results could be read by portable LF readers or smartphones. These advantages offer FLFIA as a promising technology for pathogen detection in food and water.Entities:
Keywords: E. coli O157:H7; Fluorescent lateral flow; Food safety; Graphene Oxide (PubChem CID: 124202900); Graphene oxide; Minced beef; Phosphate buffered saline (PubChem CID: 24978514); Streptavidin-conjugated CdSe/ZnS quantum dots (Qdot™ 655) (PubChem CID: 121237577); Tween-20 (PubChem CID: 443314); Water quality
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Year: 2019 PMID： 31253343 DOI： 10.1016/j.foodchem.2019.124965
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514