Literature DB >> 31250309

Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana.

Qiong Zhao1, Ying Zhu2, Wenhan Cao2, Jinbo Shen2, Yong Cui2, Shuxian Huang2, Liwen Jiang2,3.   

Abstract

FREE1 (FYVE domain protein required for endosomal sorting 1), a newly identified component of endosomal sorting complex required for transport I (ESCRT I), plays multiple roles in regulating protein trafficking and organelle biogenesis in Arabidopsis. Similar to other ESCRT components, FREE1 is essential for plant growth and development because free1 mutant is seedling lethal. To identify key components that genetically interact with FREE1, we performed forward genetic suppressor screening using a dexamethasone (DEX)-inducible FREE1-RNAi line. Here we describe the detailed protocol of identifying novel FREE1 regulators using an inducible FREE1-RNAi line for the genetic suppressor screen. This protocol represents a whole procedure for identifying ESCRT genetic interactors in Arabidopsis thaliana.

Entities:  

Keywords:  EMS mutagenesis; ESCRT; FREE1; Forward genetic screening; Inducible; Mapping; RNAi; Suppressor

Mesh：

Substances：

Year:  2019        PMID： 31250309     DOI： 10.1007/978-1-4939-9492-2_20

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

Review 1.  Biogenesis of Plant Prevacuolar Multivesicular Bodies.

Authors:  Yong Cui; Jinbo Shen; Caiji Gao; Xiaohong Zhuang; Junqi Wang; Liwen Jiang
Journal:  Mol Plant       Date:  2016-02-02       Impact factor: 13.164

2.  A unique plant ESCRT component, FREE1, regulates multivesicular body protein sorting and plant growth.

Authors:  Caiji Gao; Ming Luo; Qiong Zhao; Renzhi Yang; Yong Cui; Yonglun Zeng; Jun Xia; Liwen Jiang
Journal:  Curr Biol       Date:  2014-10-16       Impact factor: 10.834

3.  Dual roles of an Arabidopsis ESCRT component FREE1 in regulating vacuolar protein transport and autophagic degradation.

Authors:  Caiji Gao; Xiaohong Zhuang; Yong Cui; Xi Fu; Yilin He; Qiong Zhao; Yonglun Zeng; Jinbo Shen; Ming Luo; Liwen Jiang
Journal:  Proc Natl Acad Sci U S A       Date:  2015-01-26       Impact factor: 11.205

4.  FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis.

Authors:  Cornelia Kolb; Marie-Kristin Nagel; Kamila Kalinowska; Jörg Hagmann; Mie Ichikawa; Franziska Anzenberger; Angela Alkofer; Masa H Sato; Pascal Braun; Erika Isono
Journal:  Plant Physiol       Date:  2015-02-19       Impact factor: 8.340

5.  Fast-suppressor screening for new components in protein trafficking, organelle biogenesis and silencing pathway in Arabidopsis thaliana using DEX-inducible FREE1-RNAi plants.

Authors:  Qiong Zhao; Caiji Gao; PoShing Lee; Lin Liu; Shaofang Li; Tangjin Hu; Jinbo Shen; Shuying Pan; Hao Ye; Yunru Chen; Wenhan Cao; Yong Cui; Peng Zeng; Sheng Yu; Yangbin Gao; Liang Chen; Beixin Mo; Xin Liu; Shi Xiao; Yunde Zhao; Silin Zhong; Xuemei Chen; Liwen Jiang
Journal:  J Genet Genomics       Date:  2015-05-14       Impact factor: 4.275

Review 6.  Plant ESCRT Complexes: Moving Beyond Endosomal Sorting.

Authors:  Caiji Gao; Xiaohong Zhuang; Jinbo Shen; Liwen Jiang
Journal:  Trends Plant Sci       Date:  2017-08-31       Impact factor: 18.313

7.  The Sequence Alignment/Map format and SAMtools.

Authors:  Heng Li; Bob Handsaker; Alec Wysoker; Tim Fennell; Jue Ruan; Nils Homer; Gabor Marth; Goncalo Abecasis; Richard Durbin
Journal:  Bioinformatics       Date:  2009-06-08       Impact factor: 6.937

8.  A BAR-domain protein SH3P2, which binds to phosphatidylinositol 3-phosphate and ATG8, regulates autophagosome formation in Arabidopsis.

Authors:  Xiaohong Zhuang; Hao Wang; Sheung Kwan Lam; Caiji Gao; Xiangfeng Wang; Yi Cai; Liwen Jiang
Journal:  Plant Cell       Date:  2013-11-18       Impact factor: 11.277

9.  Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling.

Authors:  Ana M Calcagno-Pizarelli; América Hervás-Aguilar; Antonio Galindo; Juan F Abenza; Miguel A Peñalva; Herbert N Arst
Journal:  J Cell Sci       Date:  2011-12-01       Impact factor: 5.285

10.  Fast and accurate short read alignment with Burrows-Wheeler transform.

Authors:  Heng Li; Richard Durbin
Journal:  Bioinformatics       Date:  2009-05-18       Impact factor: 6.937
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