| Literature DB >> 31250299 |
Shai Adar-Levor1, Inna Goliand1, Michael Elbaum2, Natalie Elia3.
Abstract
The ESCRT machinery mediates scission of the intercellular bridge that connects two daughter cells at the end of cytokinesis. Structured illumination microscopy (SIM) and cryo-soft-X-ray tomography (cryo-SXT) have been used in recent years to study the topology of ESCRT-driven cytokinetic abscission. These studies revealed that the intercellular bridge is occupied by cortical rings and spiral-like filaments and that ESCRTs form ring-like structures in this region during abscission. In this chapter, we provide two protocols: a protocol for determining the spatial organization of specific ESCRT components at the intercellular bridge using SIM and a protocol for resolving the ultrastructural organization of cortical filaments at the intercellular bridge using cryo-SXT.Entities:
Keywords: Cell division; Correlative imaging; Cryo; Cytokinesis; ESCRT; ESCRT-III; Fluorescence imaging; Immunostaining; Membrane fission; Soft-X-ray; Super-resolution microscopy; Synchronization; Tomography
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Year: 2019 PMID： 31250299 DOI： 10.1007/978-1-4939-9492-2_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745