Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Live Imaging of ESCRT Proteins in Microfluidically Isolated Hippocampal Axons.

Literature DB >> 31250298

Live Imaging of ESCRT Proteins in Microfluidically Isolated Hippocampal Axons.

Veronica Birdsall¹, Jose C Martinez², Lisa Randolph¹, Ulrich Hengst^3,4, Clarissa L Waites^5,6.

Abstract

Live imaging of microfluidically isolated axons permits study of the dynamic behavior of fluorescently tagged proteins and vesicles in these neuronal processes. We use this technique to study the motility and transport of ESCRT proteins in axons of primary hippocampal neurons. This chapter details the preparation of microfluidic chambers, as well as the seeding, fluidic isolation, and lentiviral transduction of hippocampal neurons in these chambers, optimized for the study of ESCRT protein dynamics.

Entities: Chemical

Keywords: Axonal isolation; Axonal transport; ESCRT; Hippocampal neuron; Live cell imaging; Microfluidic; Primary neuronal culture

Mesh：

Substances：

Year: 2019 PMID： 31250298 PMCID： PMC8882341 DOI： 10.1007/978-1-4939-9492-2_9

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

9 in total

1. The coiled-coil protein shrub controls neuronal morphogenesis in Drosophila.

Authors: Neal T Sweeney; Jay E Brenman; Yuh Nung Jan; Fen-Biao Gao
Journal: Curr Biol Date: 2006-05-23 Impact factor: 10.834

2. Microfluidic culture platform for neuroscience research.

Authors: Jeong Won Park; Behrad Vahidi; Anne M Taylor; Seog Woo Rhee; Noo Li Jeon
Journal: Nat Protoc Date: 2006 Impact factor: 13.491

3. Oligodendroglial deletion of ESCRT-I component TSG101 causes spongiform encephalopathy.

Authors: Will P Walker; Abby Oehler; Aimee L Edinger; Kay-Uwe Wagner; Teresa M Gunn
Journal: Biol Cell Date: 2016-08-23 Impact factor: 4.458

4. A transgenic mouse expressing CHMP2Bintron5 mutant in neurons develops histological and behavioural features of amyotrophic lateral sclerosis and frontotemporal dementia.

Authors: Aurélia Vernay; Ludivine Therreau; Béatrice Blot; Valérie Risson; Sylvie Dirrig-Grosch; Robin Waegaert; Thiebault Lequeu; François Sellal; Laurent Schaeffer; Rémy Sadoul; Jean-Philippe Loeffler; Frédérique René
Journal: Hum Mol Genet Date: 2016-06-21 Impact factor: 6.150

5. Intra-axonal Synthesis of SNAP25 Is Required for the Formation of Presynaptic Terminals.

Authors: Andreia F R Batista; José C Martínez; Ulrich Hengst
Journal: Cell Rep Date: 2017-09-26 Impact factor: 9.423

Review 6. Recent advances in the genetics of amyotrophic lateral sclerosis and frontotemporal dementia: common pathways in neurodegenerative disease.

Authors: Kevin Talbot; Olaf Ansorge
Journal: Hum Mol Genet Date: 2006-10-15 Impact factor: 6.150

7. ESCRT-III dysfunction causes autophagosome accumulation and neurodegeneration.

Authors: Jin-A Lee; Anne Beigneux; S Tariq Ahmad; Stephen G Young; Fen-Biao Gao
Journal: Curr Biol Date: 2007-08-02 Impact factor: 10.834

8. Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration.

Authors: Keiichi Tamai; Masafumi Toyoshima; Nobuyuki Tanaka; Noriko Yamamoto; Yuji Owada; Hiroshi Kiyonari; Kazuko Murata; Yoshiyuki Ueno; Masao Ono; Tooru Shimosegawa; Nobuo Yaegashi; Masahiko Watanabe; Kazuo Sugamura
Journal: Am J Pathol Date: 2008-11-13 Impact factor: 4.307

9. Mutations in CHMP2B in lower motor neuron predominant amyotrophic lateral sclerosis (ALS).

Authors: Laura E Cox; Laura Ferraiuolo; Emily F Goodall; Paul R Heath; Adrian Higginbottom; Heather Mortiboys; Hannah C Hollinger; Judith A Hartley; Alice Brockington; Christine E Burness; Karen E Morrison; Stephen B Wharton; Andrew J Grierson; Paul G Ince; Janine Kirby; Pamela J Shaw
Journal: PLoS One Date: 2010-03-24 Impact factor: 3.240