| Literature DB >> 31250296 |
Jalal M Kazan1,2, Gergely L Lukacs3, Pirjo M Apaja4,5, Arnim Pause6,7.
Abstract
The endosomal sorting complexes required for transport (ESCRT) comprise a major trafficking pathway for plasma membrane proteins and are fundamental for ubiquitin-dependent cargo endocytosis. Here, we describe a method for studying the effect of ESCRT complexes on endo-lysosomal membrane trafficking and their role in receptor integrin α5β1 downregulation. Single cell fluorescence ratio image analysis (FRIA), using appropriate fluorescence probes, enables the measurement of dynamics of integrin α5β1 containing vesicles and represents a live cell-based method for studying the role of ESCRTs.Entities:
Keywords: ESCRT; FRIA; HD-PTP; Integrin α5β1; Lysosomes; Recycling; UBAP1; Vesicular pH
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Year: 2019 PMID： 31250296 DOI： 10.1007/978-1-4939-9492-2_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745