| Literature DB >> 31250295 |
Keisuke Tabata1, Atsuki Nara2, Hiroko Omori3, Eiji Morita4.
Abstract
Many enveloped viruses utilize the cellular ESCRT pathway for budding, even flaviviruses, which form viral particles inside replication organelles derived from the endoplasmic reticulum (ER). In this section, we introduce methods for detecting several ESCRT subunit proteins in virus-infected cells by immunofluorescence microscopy and immunoelectron microscopy (immuno-EM). We also introduce a new method; correlative light microscopy and electron microscopy (CLEM), which allows the observation of target structures with both high-resolution EM and fluorescence labeling.Entities:
Keywords: Conventional transmission EM (TEM); Correlative light microscopy and electron microscopy (CLEM); Dengue virus (DENV); Fluorescence light microscopy; Immunoelectron microscopy; Japanese encephalitis virus (JEV); Positive strand RNA virus
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Year: 2019 PMID： 31250295 DOI： 10.1007/978-1-4939-9492-2_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745