A comparison of uterine contraction induced by PGE1 and oxytocin in Ca-free solution.

The contraction of the rat uterus incubated in Ca-free EDTA-containing solution in response to PGE1, oxytocin and vanadate has been investigated in order to examine the mechanism of the release of Ca from intracellular stores. The results obtained show that PGE1 evoked a sustained contraction the magnitude of which diminishes slightly after successive additions of PGE1 but not after long exposure to Ca-free medium. Oxytocin induced two different contractions: one of them was transient and observed only after incubating for 5 min in Ca-free solution; the other remained constant during prolonged incubation in Ca-free medium. Vanadate, an inhibitor of Ca-ATPase, induced sustained contraction after prolonged exposure to Ca-free medium, and isoprenaline, which stimulates Ca re-uptake by intracellular organelles, counteracted the sustained contractile response induced by the three agonists.