Improved Surface Functionalization and Characterization of Membrane-Targeted Semiconductor Voltage Nanosensors.

Type-II ZnSe/CdS voltage-sensing seeded nanorods (vsNRs) were functionalized with α-helical peptides and zwitterionic-decorated lipoic acids (zw-LAs). Specific membrane targeting with high loading efficiency and minimal nonspecific binding was achieved. These vsNRs display quantum yield (QY) modulation as a function of membrane potential (MP) changes, as demonstrated at the ensemble level for (i) vesicles treated with valinomycin and (ii) wild-type HEK cells under alternating buffers with different [K+]. ΔF/F of ∼ 1% was achieved.