| Literature DB >> 31237533 |
Haifa Bennour1,2, Imene Fodha1,2, Asma Bouazizi1,2, Meriam Ben Hamida-Rebaï1,2, Amira Jerbi1,2, Mouna Ben Hadj Fredj1,3, Samia Lakhal1,2, Ameni Dhiflaoui1,2, Shada Abdelberi1,2, Fairouz Abbassi1,2, Noureddine Boujaafar4, Akila Fathallah5,6, Saoussen Abroug7, Monia Khlifa8, Abdelhalim Trabelsi1,2.
Abstract
The aim of the present study was to report the molecular characterization of human group A rotaviruses (RVAs) circulating in Tunisia. Stool specimens were collected from children under 5 years of age who had been hospitalized or were consulting for gastroenteritis in Tunisian hospitals between 2015 and 2017. All samples were screened by reverse-transcription polymerase chain reaction (RT-PCR) for the detection of the VP6 gene specific for RVA. RVA-positive samples were further analysed for G/P genotyping by semi-nested multiplex RT-PCR. Among 454 tested samples, 72 (15.8 %) were positive for RVA. G1P[8] was the most prevalent detected strain (41.7%), followed by G9P[8] (32.8%), G2P[4] (7.5%), G12P[8] (7.5%), G1P[6] (3.0%), G2P[8] (1.5%) and G3P[8] (1.5%), with mixed infections in 4.5 % of cases. In the absence of a national anti-rotavirus vaccination strategy, RVAs remain the primary aetiological agent for gastroenteritis in Tunisian children.Entities:
Keywords: Tunisia; VP4 genotype; VP7 genotype; children; gastroenteritis; group A rotavirus
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Year: 2019 PMID： 31237533 DOI： 10.1099/jmm.0.001031
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472