Joshua H Gordon1, Michael J LaMonte2, Robert J Genco3, Jiwei Zhao4, Lu Li5, Kathleen M Hovey1, Maria Tsompana6, Michael J Buck6, Christopher A Andrews7, Daniel I Mcskimming8, Wei Zheng5, Yijun Sun5, Jean Wactawski-Wende1. 1. Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, 273 Farber Hall, 3435 Main Street, Buffalo, NY, 14214, USA. 2. Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, 273 Farber Hall, 3435 Main Street, Buffalo, NY, 14214, USA. mlamonte@buffalo.edu. 3. Department of Oral Biology, School of Dental Medicine, UB Microbiome Center, University at Buffalo, Buffalo, NY, USA. 4. Department of Biostatistics, School of Public Health and Health Professions, University at Buffalo, Buffalo, USA. 5. Department of Computer and Engineering Science, NY State Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, Buffalo, NY, USA. 6. Department of Biochemistry, NY State Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, Buffalo, NY, USA. 7. Department of Ophthalmology, University of Michigan Medical School, Ann Arbor, MI, USA. 8. Genome Environment, and Microbiome Center of Excellence, University at Buffalo, Buffalo, NY, USA.
Abstract
INTRODUCTION: A possible role of the oral microbiome, specifically oral nitrate reducing flora, in blood pressure (BP) homeostasis, if proven etiologic in nature, could lead to novel mechanism-based therapy to improve hypertension prevention and control. AIM: This cross-sectional study characterized and compared the oral microbiome between four study groups based on BP status among 446 postmenopausal women aged 53-82 years. METHODS: Three study groups were not taking hypertension medication and were separated based on BP, as follows: normal BP (systolic < 120 and diastolic < 80; N = 179), elevated BP/Stage I hypertension (systolic 120-139 or diastolic 80-90; N = 106), Stage II hypertension (systolic > 140 or diastolic > 90; N = 42). The forth group consisted of anyone taking hypertension medications, regardless of BP (N = 119). Subgingival microbiome composition was determined using 16S rRNA sequencing with the Illumina MiSeq platform. Kruskal-Wallis tests were used to compare species-level relative abundance of bacterial operational taxonomic units across the four groups. RESULTS: Sixty-five bacterial species demonstrated significant differences in relative abundance in women with elevated BP or using hypertension medication as compared to those with normal BP. After correction for multiple testing, two species, Prevotella oral (species 317) and Streptococcus oralis, remained significant and were lower in abundance among women taking antihypertension medications compared to those with normal BP (corrected P < 0.05). CONCLUSIONS: These data provide novel description of oral subgingival bacteria grouped according to BP status. Additional larger studies including functional analysis and prospective designs will help further assess the potential role of the oral microbiome in BP regulation and hypertension.
INTRODUCTION: A possible role of the oral microbiome, specifically oral nitrate reducing flora, in blood pressure (BP) homeostasis, if proven etiologic in nature, could lead to novel mechanism-based therapy to improve hypertension prevention and control. AIM: This cross-sectional study characterized and compared the oral microbiome between four study groups based on BP status among 446 postmenopausal women aged 53-82 years. METHODS: Three study groups were not taking hypertension medication and were separated based on BP, as follows: normal BP (systolic < 120 and diastolic < 80; N = 179), elevated BP/Stage I hypertension (systolic 120-139 or diastolic 80-90; N = 106), Stage II hypertension (systolic > 140 or diastolic > 90; N = 42). The forth group consisted of anyone taking hypertension medications, regardless of BP (N = 119). Subgingival microbiome composition was determined using 16S rRNA sequencing with the Illumina MiSeq platform. Kruskal-Wallis tests were used to compare species-level relative abundance of bacterial operational taxonomic units across the four groups. RESULTS: Sixty-five bacterial species demonstrated significant differences in relative abundance in women with elevated BP or using hypertension medication as compared to those with normal BP. After correction for multiple testing, two species, Prevotella oral (species 317) and Streptococcus oralis, remained significant and were lower in abundance among women taking antihypertension medications compared to those with normal BP (corrected P < 0.05). CONCLUSIONS: These data provide novel description of oral subgingival bacteria grouped according to BP status. Additional larger studies including functional analysis and prospective designs will help further assess the potential role of the oral microbiome in BP regulation and hypertension.
Entities:
Keywords:
Blood pressure; Epidemiology; Hypertension; Mechanism; Microbiology; Microbiome; Women
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