Literature DB >> 3123435

Monoclonal antibody highly sensitive for the detection of ras p21 in immunoblotting analysis.

T Kanai1, S Hirohashi, M Noguchi, Y Shimoyama, Y Shimosato, S Noguchi, S Nishimura, O Abe.   

Abstract

Four murine monoclonal antibodies (NCC-RAS-001, -004, -005, -017) reactive with ras p21 were produced by using recombinant c-Ha-ras p21 as an immunogen. Among these antibodies, NCC-RAS-004 was extremely sensitive when used in immunoblotting analysis, facilitating semiquantitative detection of c-Ha-, c-Ki- and N-ras p21 in cell and tissue lysates. In cells carrying a point-mutationally activated ras, p21 with abnormal mobility upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis was clearly detected.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3123435

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  11 in total

1.  Stereochemistry-dependent inhibition of RAS farnesylation by farnesyl phosphonic acids.

Authors:  R J Hohl; K A Lewis; D M Cermak; D F Wiemer
Journal:  Lipids       Date:  1998-01       Impact factor: 1.880

2.  Requirement of Ras for the activation of mitogen-activated protein kinase by calcium influx, cAMP, and neurotrophin in hippocampal neurons.

Authors:  N Iida; K Namikawa; H Kiyama; H Ueno; S Nakamura; S Hattori
Journal:  J Neurosci       Date:  2001-09-01       Impact factor: 6.167

3.  Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade.

Authors:  Y Ohba; N Mochizuki; K Matsuo; S Yamashita; M Nakaya; Y Hashimoto; M Hamaguchi; T Kurata; K Nagashima; M Matsuda
Journal:  Mol Cell Biol       Date:  2000-08       Impact factor: 4.272

4.  Geranylgeranyl-pyrophosphate, an isoprenoid of mevalonate cascade, is a critical compound for rat primary cultured cortical neurons to protect the cell death induced by 3-hydroxy-3-methylglutaryl-CoA reductase inhibition.

Authors:  T Tanaka; I Tatsuno; D Uchida; I Moroo; H Morio; S Nakamura; Y Noguchi; T Yasuda; M Kitagawa; Y Saito; A Hirai
Journal:  J Neurosci       Date:  2000-04-15       Impact factor: 6.167

5.  Thymoma: tumour type related to expression of epidermal growth factor (EGF), EGF-receptor, p53, v-erb B and ras p21.

Authors:  Y Hayashi; N Ishii; C Obayashi; K Jinnai; K Hanioka; Y Imai; H Itoh
Journal:  Virchows Arch       Date:  1995       Impact factor: 4.064

6.  The AMeX method: a multipurpose tissue-processing and paraffin-embedding method. Extraction of protein and application to immunoblotting.

Authors:  Y Sato; K Mukai; S Furuya; T Kameya; S Hirohashi
Journal:  Am J Pathol       Date:  1992-04       Impact factor: 4.307

7.  Involvement of prolonged ras activation in thrombopoietin-induced megakaryocytic differentiation of a human factor-dependent hematopoietic cell line.

Authors:  I Matsumura; K Nakajima; H Wakao; S Hattori; K Hashimoto; H Sugahara; T Kato; H Miyazaki; T Hirano; Y Kanakura
Journal:  Mol Cell Biol       Date:  1998-07       Impact factor: 4.272

8.  Specific proto-oncogenic tyrosine kinases of src family are enriched in cell-to-cell adherens junctions where the level of tyrosine phosphorylation is elevated.

Authors:  S Tsukita; K Oishi; T Akiyama; Y Yamanashi; T Yamamoto; S Tsukita
Journal:  J Cell Biol       Date:  1991-05       Impact factor: 10.539

9.  Signal transduction pathways through TRK-A and TRK-B receptors in human neuroblastoma cells.

Authors:  T Sugimoto; H Kuroda; Y Horii; H Moritake; T Tanaka; S Hattori
Journal:  Jpn J Cancer Res       Date:  2001-02

10.  Reversible suppression by nalidixic acid of anchorage-independent growth of mouse cells transformed by 3-methylcholanthrene or an activated c-Ha-ras gene.

Authors:  M Kaneko; J Horikoshi
Journal:  Br J Cancer       Date:  1989-12       Impact factor: 7.640
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.