Literature DB >> 3123368

Analysis of an anti-progesterone antibody: variable crystal morphology of the Fab' and steroid-Fab' complexes.

E A Stura1, J H Arevalo, A Feinstein, R B Heap, M J Taussig, I A Wilson.   

Abstract

Anti-progesterone monoclonal antibodies are being used for structural studies of antibody-antigen interaction, for their ability to block pregnancy shortly after fertilization, and for hormone immunoassay. A mouse anti-progesterone monoclonal Fab' fragment has been crystallized in its native form and co-crystallized with seven different, but structurally related, steroids. The crystals show interesting preferences in their crystal morphology, depending on the bound steroid ligand. The X-ray crystallographic analysis of this Fab', complexed with a series of related steroid ligands, should reveal details of the chemistry of antibody-antigen union and provide insights into how steroids interact with proteins.

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Year:  1987        PMID： 3123368      PMCID： PMC1454165     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  33 in total

Review 1.  Mechanism of steroid binding to serum proteins.

Authors:  U Westphal; S D Stroupe; S L Chen; G B Harding
Journal:  J Toxicol Environ Health       Date:  1978 Mar-May

2.  Crystallization of human placental estradiol 17beta-dehydrogenase. A new method for crystallizing labile enzymes.

Authors:  C C Chin; J B Dence; J C Warren
Journal:  J Biol Chem       Date:  1976-06-25       Impact factor: 5.157

Review 3.  Molecular conformation and protein binding affinity of progestins.

Authors:  W L Duax; V Cody; J F Griffin; D C Rohrer; C M Weeks
Journal:  J Toxicol Environ Health       Date:  1978 Mar-May

Review 4.  Three-dimensional structure of immunoglobulins.

Authors:  L M Amzel; R J Poljak
Journal:  Annu Rev Biochem       Date:  1979       Impact factor: 23.643

5.  Structural diversity among anti-p-azophenylarsonate monoclonal antibodies from A/J mice; comparison of Id- and Id+ sequences.

Authors:  M N Margolies; A Marshak-Rothstein; M L Gefter
Journal:  Mol Immunol       Date:  1981-12       Impact factor: 4.407

6.  Progesterone monoclonal antibody blocks pregnancy in mice.

Authors:  L J Wright; A Feinstein; R B Heap; J C Saunders; R C Bennett; M Y Wang
Journal:  Nature       Date:  1982-02-04       Impact factor: 49.962

7.  X-ray crystallographic analysis of a progesterone-binding protein. The C2221 crystal form of oxidized uteroglobin at 2.2 A resolution.

Authors:  J P Mornon; F Fridlansky; R Bally; E Milgrom
Journal:  J Mol Biol       Date:  1980-03-15       Impact factor: 5.469

8.  Crystallographic refinement and atomic models of the intact immunoglobulin molecule Kol and its antigen-binding fragment at 3.0 A and 1.0 A resolution.

Authors:  M Marquart; J Deisenhofer; R Huber; W Palm
Journal:  J Mol Biol       Date:  1980-08-25       Impact factor: 5.469

9.  Preliminary refinement and structural analysis of the Fab fragment from human immunoglobulin new at 2.0 A resolution.

Authors:  F A Saul; L M Amzel; R J Poljak
Journal:  J Biol Chem       Date:  1978-01-25       Impact factor: 5.157

10.  Refinement of the C222(1) crystal form of oxidized uteroglobin at 1.34 A resolution.

Authors:  I Morize; E Surcouf; M C Vaney; Y Epelboin; M Buehner; F Fridlansky; E Milgrom; J P Mornon
Journal:  J Mol Biol       Date:  1987-04-20       Impact factor: 5.469
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  6 in total

1.  In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library.

Authors:  H Gram; L A Marconi; C F Barbas; T A Collet; R A Lerner; A S Kang
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

2.  Blocking of pregnancy in mice by immunization with anti-idiotype directed against monoclonal anti-progesterone antibody.

Authors:  M W Wang; R B Heap; M J Taussig
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

3.  Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.

Authors:  Mingyue He; Maureen Hamon; Hong Liu; Adam L Corper; Michael J Taussig
Journal:  Protein Sci       Date:  2006-08-01       Impact factor: 6.725

4.  Induction of anti-progesterone immunity and pregnancy blocking by anti-progesterone anti-idiotypes. Variable efficacy of polyclonal Ab2 antibodies directed against a panel of closely related Ab1 antibodies.

Authors:  M W Wang; M J Sims; P R Symington; A S Humphreys; M J Taussig
Journal:  Immunology       Date:  1991-07       Impact factor: 7.397

5.  Blot-sequencing of antibodies: application to analysis of V gene usage among anti-steroid monoclonal antibodies.

Authors:  M J Taussig; P R Symington; M Woods; M J Sims; D Beale; A S Humphreys; A J Northrop; P J Barker; N S Huskisson; J Coley
Journal:  Immunology       Date:  1991-04       Impact factor: 7.397

6.  Characterization of a progesterone-binding, three-domain antibody fragment (VH/K) expressed in Escherichia coli.

Authors:  M He; A S Kang; M Hamon; A S Humphreys; M Gani; M J Taussig
Journal:  Immunology       Date:  1995-04       Impact factor: 7.397
  6 in total

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