| Literature DB >> 31230276 |
Paula Tríbulo1, Rocío Melissa Rivera2, Martha Sofia Ortega Obando2, Elizabeth Ann Jannaman1, Peter J Hansen3.
Abstract
A protocol for production of bovine embryos from oocytes collected from ovaries obtained from an abattoir is described. The protocol includes methods for in vitro maturation of oocytes, capacitation of sperm, fertilization, and development of the resultant embryos to the blastocyst stage. The protocol can be easily modified to use oocytes collected by ultrasound-guided follicular aspiration.Entities:
Keywords: Bovine; Culture; Embryo; In vitro fertilization; In vitro maturation
Mesh:
Year: 2019 PMID: 31230276 DOI: 10.1007/978-1-4939-9566-0_8
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745