Marcin Olek1, Jacek Kasperski1, Dariusz Skaba2, Rafał Wiench2, Grzegorz Cieślar3, Aleksandra Kawczyk-Krupka4. 1. School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Department of Prosthetic Dentistry, Chair of Prosthetics and Dental Materials, Akademicki Sq. 17, 41-902 Bytom, Poland. 2. School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Department of Periodontal Diseases and Oral Mucosa Diseases, Traugutta Sq. 2, 41-800 Zabrze, Poland. 3. School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Department of Internal Medicine, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Batorego Street 15, 41-902 Bytom, Poland. 4. School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Department of Internal Medicine, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Batorego Street 15, 41-902 Bytom, Poland. Electronic address: akawczyk@gmail.com.
Abstract
BACKGROUND: Oral cavity squamous cell carcinoma is a common cancer of the head and neck region. Due to the frequency of diagnoses, high rate of mortality, mutilating nature of classic therapy and numerous complications, new methods of treatment are being sought. One promising solution for treatment which is utilized in many fields of oncology is photodynamic therapy. The purpose of this article is to present a general overview of the use of photodynamic therapy in preclinical in vitro studies. MATERIALS AND METHODS: A literature search for articles corresponding to the topic of this review was performed using the PubMed and MEDLINE databases using the following keywords: 'oral cavity squamous cell carcinoma,' 'photodynamic therapy,' 'photosensitizer(s),' 'in vitro,' 'cell culture(s),' 'spheroids,' 'CAM model', and 'in vivo.' RESULTS: Most of the previous work found in the literature search concerns research on the use of various photosensitizers and the determination of their level of phototoxicity against cell lines. CONCLUDING REMARKS: Research on the photodynamic effect in cell lines may be useful in establishing the mechanisms and effectiveness of the photodynamic method as a starting point for clinical trials. Studies on spheroidal models allows for testing photodynamic therapy under more clinical-like conditions. The Chick Chorioallantoic Membrane Assay provides information about the vascular changes after treatment.
BACKGROUND: Oral cavity squamous cell carcinoma is a common cancer of the head and neck region. Due to the frequency of diagnoses, high rate of mortality, mutilating nature of classic therapy and numerous complications, new methods of treatment are being sought. One promising solution for treatment which is utilized in many fields of oncology is photodynamic therapy. The purpose of this article is to present a general overview of the use of photodynamic therapy in preclinical in vitro studies. MATERIALS AND METHODS: A literature search for articles corresponding to the topic of this review was performed using the PubMed and MEDLINE databases using the following keywords: 'oral cavity squamous cell carcinoma,' 'photodynamic therapy,' 'photosensitizer(s),' 'in vitro,' 'cell culture(s),' 'spheroids,' 'CAM model', and 'in vivo.' RESULTS: Most of the previous work found in the literature search concerns research on the use of various photosensitizers and the determination of their level of phototoxicity against cell lines. CONCLUDING REMARKS: Research on the photodynamic effect in cell lines may be useful in establishing the mechanisms and effectiveness of the photodynamic method as a starting point for clinical trials. Studies on spheroidal models allows for testing photodynamic therapy under more clinical-like conditions. The Chick Chorioallantoic Membrane Assay provides information about the vascular changes after treatment.
Authors: Marcin Olek; Agnieszka Machorowska-Pieniążek; Wojciech Stós; Janusz Kalukin; Dorota Bartusik-Aebisher; David Aebisher; Grzegorz Cieślar; Aleksandra Kawczyk-Krupka Journal: Pharmaceutics Date: 2021-05-14 Impact factor: 6.321