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Literature DB >> 31225754

Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13.

Omar O Abudayyeh^1,2,3,4,5, Jonathan S Gootenberg^1,2,3,4,6, Max J Kellner¹, Feng Zhang^1,2,3,4.

Abstract

Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identification. Here, we use a modified version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (∼15 min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This field-ready SHERLOCK platform with color-based lateral flow readout can be applied for detection and quantitation of genes in a range of agricultural applications.

Entities: Chemical Species

Mesh：

Substances：

Year: 2019 PMID： 31225754 PMCID： PMC7001462 DOI： 10.1089/crispr.2019.0011

Source DB: PubMed Journal: CRISPR J ISSN： 2573-1599

28 in total

Review 1. Molecular Soybean-Pathogen Interactions.

Authors: Steven A Whitham; Mingsheng Qi; Roger W Innes; Wenbo Ma; Valéria Lopes-Caitar; Tarek Hewezi
Journal: Annu Rev Phytopathol Date: 2016-01-17 Impact factor: 13.078

2. Type III CRISPR-Cas systems produce cyclic oligoadenylate second messengers.

Authors: Ole Niewoehner; Carmela Garcia-Doval; Jakob T Rostøl; Christian Berk; Frank Schwede; Laurent Bigler; Jonathan Hall; Luciano A Marraffini; Martin Jinek
Journal: Nature Date: 2017-07-19 Impact factor: 49.962

Review 3. Altering the Gut Microbiome of Cattle: Considerations of Host-Microbiome Interactions for Persistent Microbiome Manipulation.

Authors: Brooke A Clemmons; Brynn H Voy; Phillip R Myer
Journal: Microb Ecol Date: 2018-07-22 Impact factor: 4.552

4. RNA editing with CRISPR-Cas13.

Authors: David B T Cox; Jonathan S Gootenberg; Omar O Abudayyeh; Brian Franklin; Max J Kellner; Julia Joung; Feng Zhang
Journal: Science Date: 2017-10-25 Impact factor: 47.728

5. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector.

Authors: Omar O Abudayyeh; Jonathan S Gootenberg; Silvana Konermann; Julia Joung; Ian M Slaymaker; David B T Cox; Sergey Shmakov; Kira S Makarova; Ekaterina Semenova; Leonid Minakhin; Konstantin Severinov; Aviv Regev; Eric S Lander; Eugene V Koonin; Feng Zhang
Journal: Science Date: 2016-06-02 Impact factor: 47.728

6. RNA targeting with CRISPR-Cas13.

Authors: Omar O Abudayyeh; Jonathan S Gootenberg; Patrick Essletzbichler; Shuo Han; Julia Joung; Joseph J Belanto; Vanessa Verdine; David B T Cox; Max J Kellner; Aviv Regev; Eric S Lander; Daniel F Voytas; Alice Y Ting; Feng Zhang
Journal: Nature Date: 2017-10-04 Impact factor: 49.962

7. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.

Authors: Jian Ye; George Coulouris; Irena Zaretskaya; Ioana Cutcutache; Steve Rozen; Thomas L Madden
Journal: BMC Bioinformatics Date: 2012-06-18 Impact factor: 3.169

8. GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use.

Authors: Guy Kiddle; Patrick Hardinge; Neil Buttigieg; Olga Gandelman; Clint Pereira; Cathal J McElgunn; Manuela Rizzoli; Rebecca Jackson; Nigel Appleton; Cathy Moore; Laurence C Tisi; James A H Murray
Journal: BMC Biotechnol Date: 2012-04-30 Impact factor: 2.563

9. Multiplex, Rapid, and Sensitive Isothermal Detection of Nucleic-Acid Sequence by Endonuclease Restriction-Mediated Real-Time Multiple Cross Displacement Amplification.

Authors: Yi Wang; Yan Wang; Lu Zhang; Dongxin Liu; Lijuan Luo; Hua Li; Xiaolong Cao; Kai Liu; Jianguo Xu; Changyun Ye
Journal: Front Microbiol Date: 2016-05-18 Impact factor: 5.640

Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13.

Review 1. Molecular Soybean-Pathogen Interactions.

2. Type III CRISPR-Cas systems produce cyclic oligoadenylate second messengers.

Review 3. Altering the Gut Microbiome of Cattle: Considerations of Host-Microbiome Interactions for Persistent Microbiome Manipulation.

4. RNA editing with CRISPR-Cas13.

5. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector.

6. RNA targeting with CRISPR-Cas13.

7. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.

8. GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use.

9. Multiplex, Rapid, and Sensitive Isothermal Detection of Nucleic-Acid Sequence by Endonuclease Restriction-Mediated Real-Time Multiple Cross Displacement Amplification.

10. Development of methodologies for virus detection in soybean and wheat seeds.

1. Programmable RNA targeting with the single-protein CRISPR effector Cas7-11.

2. Figure of Merit for CRISPR-Based Nucleic Acid-Sensing Systems: Improvement Strategies and Performance Comparison.

Review 3. Exploration of Plant-Microbe Interactions for Sustainable Agriculture in CRISPR Era.

Review 4. Harnessing the potential of CRISPR-based platforms to advance the field of hospital medicine.

Review 5. Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology.

6. The expanded development and application of CRISPR system for sensitive nucleotide detection.

7. CRISPR-Cas13d mediates robust RNA virus interference in plants.

8. A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila.

9. Rapid and accurate species identification for ecological studies and monitoring using CRISPR-based SHERLOCK.

Review 10. Functional Features and Current Applications of the RNA-Targeting Type VI CRISPR-Cas Systems.