Khine Mar Oo1, Tin Ohn Myat1,2, Wah Win Htike1, Ambarish Biswas3, Rachel F Hannaway3, David R Murdoch4, John A Crump2, James E Ussher3,5. 1. Department of Microbiology, University of Medicine 1, No. 245 Myoma Kyaung Street, Lanmadaw Township, Yangon, Myanmar. 2. Centre for International Health, University of Otago, Dunedin, New Zealand. 3. Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand. 4. Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand. 5. Southern Community Laboratories, Dunedin, New Zealand.
Abstract
BACKGROUND: Enteric fever is common in southeast Asia. However, there is little information on the circulating Salmonella enterica strains causing enteric fever in Myanmar. METHODS: We performed antimicrobial susceptibility testing and whole genome sequencing on S. enterica bloodstream isolates from febrile patients aged ≥12 y attending two hospitals in Yangon, Myanmar, from 5 October 2015 through 4 October 2016. We identified the serovar of S. enterica, determined antimicrobial susceptibility and the molecular mechanisms of resistance. We analysed phylogenetic relationships among Myanmar S. enterica isolates and those with isolates from neighbouring countries. RESULTS: Of 73 S. enterica isolated, 39 (53%) were serovar Typhi and 34 (47%) were Paratyphi A. All isolates were susceptible to ampicillin, chloramphenicol and trimethoprim-sulfamethoxazole but resistant to ciprofloxacin. We identified mutations in chromosomal genes gyrA, gyrB and parC as responsible for fluoroquinolone resistance. All S. enterica Typhi isolates were of 4.3.1 subclade (formerly known as H58) and formed two closely related genotypic clusters; both clusters were most closely related to isolates from India from 2012. All S. enterica Paratyphi A were lineage C, clade C4 and were closely related. CONCLUSION: Our study describes currently circulating S. enterica serovars in Myanmar, the genetic basis of their antimicrobial resistance and provides a genotypic framework for epidemiologic study.
BACKGROUND:Enteric fever is common in southeast Asia. However, there is little information on the circulating Salmonella enterica strains causing enteric fever in Myanmar. METHODS: We performed antimicrobial susceptibility testing and whole genome sequencing on S. enterica bloodstream isolates from febrile patients aged ≥12 y attending two hospitals in Yangon, Myanmar, from 5 October 2015 through 4 October 2016. We identified the serovar of S. enterica, determined antimicrobial susceptibility and the molecular mechanisms of resistance. We analysed phylogenetic relationships among Myanmar S. enterica isolates and those with isolates from neighbouring countries. RESULTS: Of 73 S. enterica isolated, 39 (53%) were serovar Typhi and 34 (47%) were Paratyphi A. All isolates were susceptible to ampicillin, chloramphenicol and trimethoprim-sulfamethoxazole but resistant to ciprofloxacin. We identified mutations in chromosomal genes gyrA, gyrB and parC as responsible for fluoroquinolone resistance. All S. entericaTyphi isolates were of 4.3.1 subclade (formerly known as H58) and formed two closely related genotypic clusters; both clusters were most closely related to isolates from India from 2012. All S. enterica Paratyphi A were lineage C, clade C4 and were closely related. CONCLUSION: Our study describes currently circulating S. enterica serovars in Myanmar, the genetic basis of their antimicrobial resistance and provides a genotypic framework for epidemiologic study.
Authors: Tin Ohn Myat; Khine Mar Oo; Hla Kye Mone; Wah Win Htike; Ambarish Biswas; Rachel F Hannaway; David R Murdoch; James E Ussher; John A Crump Journal: PLoS Negl Trop Dis Date: 2020-04-30
Authors: Silvia Argimón; Corin A Yeats; Richard J Goater; Khalil Abudahab; Benjamin Taylor; Anthony Underwood; Leonor Sánchez-Busó; Vanessa K Wong; Zoe A Dyson; Satheesh Nair; Se Eun Park; Florian Marks; Andrew J Page; Jacqueline A Keane; Stephen Baker; Kathryn E Holt; Gordon Dougan; David M Aanensen Journal: Nat Commun Date: 2021-05-17 Impact factor: 14.919