Luming Yao1, Yunpeng Zhong2, Biao Wang1, Junhui Yan1, Tianlong Wu1. 1. Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China. 2. Zhengzhou Fruit Research Institute, Chinese Academy of Agriculture Sciences, Zhengzhou, China.
Abstract
BACKGROUND: Beta-aminobutyric acid (BABA) confer plant resistance to a broad spectrum of biotic and abiotic stresses. The soybean aphid (SBA), is native to eastern Asia and is a predominant insect pest of soybean. Both isoflavone and lignin pathway are important branches of the general phenylpropanoid pathway, which would be likely associated with resistance against soybean aphid. However, little is known about the role of the phenylpropanoid pathway in defense response to SBA as induced by BABA application. RESULTS: The application of BABA effectively enhanced soybean resistance against Aphis glycines, the soybean aphid. Consistent with significantly increased content of isoflavones, especially genistein, the related biosynthetic genes were upregulated by use of BABA. Lignin, another important defense component against arthropods, accumulated at a high level and four lignin biosynthesis related genes were also activated. Additionally, BABA application augmented the expression of callose synthase genes and increased callose deposition in SBA-infested seedlings. In non-caged and caged tests, SBA numbers were significantly reduced in BABA-treated seedlings. CONCLUSION: These results demonstrate that application of BABA has an obvious positive effect on soybean resistance to aphids, and this defense response partly depends on the potentiation of isoflavone biosynthesis and callose deposition.
BACKGROUND:Beta-aminobutyric acid (BABA) confer plant resistance to a broad spectrum of biotic and abiotic stresses. The soybean aphid (SBA), is native to eastern Asia and is a predominant insect pest of soybean. Both isoflavone and lignin pathway are important branches of the general phenylpropanoid pathway, which would be likely associated with resistance against soybean aphid. However, little is known about the role of the phenylpropanoid pathway in defense response to SBA as induced by BABA application. RESULTS: The application of BABA effectively enhanced soybean resistance against Aphis glycines, the soybean aphid. Consistent with significantly increased content of isoflavones, especially genistein, the related biosynthetic genes were upregulated by use of BABA. Lignin, another important defense component against arthropods, accumulated at a high level and four lignin biosynthesis related genes were also activated. Additionally, BABA application augmented the expression of callose synthase genes and increased callose deposition in SBA-infested seedlings. In non-caged and caged tests, SBA numbers were significantly reduced in BABA-treated seedlings. CONCLUSION: These results demonstrate that application of BABA has an obvious positive effect on soybean resistance to aphids, and this defense response partly depends on the potentiation of isoflavone biosynthesis and callose deposition.
Authors: Kumud Joshi; Joshua L Baumgardner; Madison MacPhail; Shailesh R Acharya; Elizabeth Blotevogel; Franck E Dayan; Punya Nachappa; Vamsi J Nalam Journal: Front Plant Sci Date: 2021-07-16 Impact factor: 5.753