| Literature DB >> 31218613 |
Aleksandra Šakanovič1, Vesna Hodnik1,2, Gregor Anderluh3.
Abstract
Surface plasmon resonance (SPR) is an established method for studying molecular interactions in real time. It allows obtaining qualitative and quantitative data on interactions of proteins with lipids or lipid membranes. In most of the approaches a lipid membrane or a membrane-mimetic surface is prepared on the surface of Biacore (GE Healthcare) sensor chips HPA or L1, and the studied protein is then injected across the surface. Here we provide an overview of SPR in protein-lipid and protein-membrane interactions, different approaches described in the literature and a general protocol for conducting an SPR experiment including lipid membranes, together with some experimental considerations.Entities:
Keywords: Biacore; Custom-made chip; Lipid membrane; Protein–membrane interactions; Sensor chip HPA; Sensor chip L1
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Year: 2019 PMID： 31218613 DOI： 10.1007/978-1-4939-9512-7_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745