Maíra Grossi-de-Sa1, Anne-Sophie Petitot2, Deisy A Xavier2,3, Maria Eugênia L Sá2,3,4, Itamara Mezzalira2,3, Magda A Beneventi2,3, Natalia F Martins3, Hugues K Baimey5, Erika V S Albuquerque3, Maria F Grossi-de-Sa3, Diana Fernandez6,7. 1. IRD, Cirad, Univ Montpellier, IPME, 911, Avenue Agropolis, 34394, Montpellier Cedex 5, France. maira.grossi-de-sa@ird.fr. 2. IRD, Cirad, Univ Montpellier, IPME, 911, Avenue Agropolis, 34394, Montpellier Cedex 5, France. 3. Embrapa Genetic Resources and Biotechnology-PqEB, Final W5 N, Brasília, DF, 70770-917, Brazil. 4. Agricultural Research Company of Minas Gerais State, EPAMIG, Uberaba, MG, Brazil. 5. Université de Parakou/Ecole Nationale Supérieure des Sciences et Techniques Agronomiques de Djougou, Parakou, Benin. 6. IRD, Cirad, Univ Montpellier, IPME, 911, Avenue Agropolis, 34394, Montpellier Cedex 5, France. diana.fernandez@ird.fr. 7. Embrapa Genetic Resources and Biotechnology-PqEB, Final W5 N, Brasília, DF, 70770-917, Brazil. diana.fernandez@ird.fr.
Abstract
MAIN CONCLUSION: This study revealed novel insights into the function of MSP18 effector during root-knot nematode parasitism in rice roots. MSP18 may modulate host immunity and enhance plant susceptibility to Meloidogyne spp. Rice (Oryza sativa) production is seriously impacted by root-knot nematodes (RKN), including Meloidogyne graminicola, Meloidogyne incognita, and Meloidogyne javanica, in upland and irrigated culture systems. Successful plant infection by RKN is likely achieved by releasing into the host cells some effector proteins to suppress the activation of immune responses. Here, we conducted a series of functional analyses to assess the role of the Meloidogyne-secreted protein (MSP) 18 from M. incognita (Mi-MSP18) during rice infection by RKN. Developmental expression profiles of M. javanica and M. graminicola showed that the MSP18 gene is up-regulated throughout nematode parasitic stages in rice. Reproduction of M. javanica and M. graminicola is enhanced in rice plants overexpressing Mi-MSP18, indicating that the Mi-MSP18 protein facilitates RKN parasitism. Transient expression assays in onion cells suggested that Mi-MSP18 is localized to the cytoplasm of the host cells. In tobacco, Mi-MSP18 suppressed the cell death induced by the INF1 elicitin, suggesting that Mi-MSP18 can interfere with the plant defense pathways. The data obtained in this study highlight Mi-MSP18 as a novel RKN effector able to enhance plant susceptibility and modulate host immunity.
MAIN CONCLUSION: This study revealed novel insights into the function of MSP18 effector during root-knot nematode parasitism in rice roots. MSP18 may modulate host immunity and enhance plant susceptibility to Meloidogyne spp. Rice (Oryza sativa) production is seriously impacted by root-knot nematodes (RKN), including Meloidogyne graminicola, Meloidogyne incognita, and Meloidogyne javanica, in upland and irrigated culture systems. Successful plant infection by RKN is likely achieved by releasing into the host cells some effector proteins to suppress the activation of immune responses. Here, we conducted a series of functional analyses to assess the role of the Meloidogyne-secreted protein (MSP) 18 from M. incognita (Mi-MSP18) during rice infection by RKN. Developmental expression profiles of M. javanica and M. graminicola showed that the MSP18 gene is up-regulated throughout nematode parasitic stages in rice. Reproduction of M. javanica and M. graminicola is enhanced in rice plants overexpressing Mi-MSP18, indicating that the Mi-MSP18 protein facilitates RKN parasitism. Transient expression assays in onion cells suggested that Mi-MSP18 is localized to the cytoplasm of the host cells. In tobacco, Mi-MSP18 suppressed the cell death induced by the INF1 elicitin, suggesting that Mi-MSP18 can interfere with the plant defense pathways. The data obtained in this study highlight Mi-MSP18 as a novel RKN effector able to enhance plant susceptibility and modulate host immunity.
Authors: Todd Wylie; John C Martin; Michael Dante; Makedonka Dautova Mitreva; Sandra W Clifton; Asif Chinwalla; Robert H Waterston; Richard K Wilson; James P McCarter Journal: Nucleic Acids Res Date: 2004-01-01 Impact factor: 16.971
Authors: C Sallaud; D Meynard; J van Boxtel; C Gay; M Bès; J P Brizard; P Larmande; D Ortega; M Raynal; M Portefaix; P B F Ouwerkerk; S Rueb; M Delseny; E Guiderdoni Journal: Theor Appl Genet Date: 2003-04-03 Impact factor: 5.699
Authors: Guozhong Huang; Ruihua Dong; Rex Allen; Eric L Davis; Thomas J Baum; Richard S Hussey Journal: Mol Plant Microbe Interact Date: 2006-05 Impact factor: 4.171
Authors: Guozhong Huang; Bingli Gao; Tom Maier; R Allen; Eric L Davis; Thomas J Baum; Richard S Hussey Journal: Mol Plant Microbe Interact Date: 2003-05 Impact factor: 4.171
Authors: E Quevillon; V Silventoinen; S Pillai; N Harte; N Mulder; R Apweiler; R Lopez Journal: Nucleic Acids Res Date: 2005-07-01 Impact factor: 16.971