OBJECTIVE: To investigate the mechanisms underlying ozone-induced inactivation of poliovirus type 1 (PV1). METHODS: We used cell culture, long-overlapping RT-PCR, and spot hybridization assays to verify and accurately locate the sites of action of ozone that cause PV1 inactivation. We also employed recombinant viral genome RNA infection models to confirm our observations. RESULTS: Our results indicated that ozone inactivated PV1 primarily by disrupting the 5'-non-coding region (5'-NCR) of the PV1 genome. Further study revealed that ozone specifically damaged the 80-124 nucleotide (nt) region in the 5'-NCR. Recombinant viral genome RNA infection models confirmed that PV1 lacking this region was non-infectious. CONCLUSION: In this study, we not only elucidated the mechanisms by which ozone induces PV1 inactivation but also determined that the 80-124 nt region in the 5'-NCR is targeted by ozone to achieve this inactivation.
OBJECTIVE: To investigate the mechanisms underlying ozone-induced inactivation of poliovirus type 1 (PV1). METHODS: We used cell culture, long-overlapping RT-PCR, and spot hybridization assays to verify and accurately locate the sites of action of ozone that cause PV1 inactivation. We also employed recombinant viral genome RNA infection models to confirm our observations. RESULTS: Our results indicated that ozone inactivated PV1 primarily by disrupting the 5'-non-coding region (5'-NCR) of the PV1 genome. Further study revealed that ozone specifically damaged the 80-124 nucleotide (nt) region in the 5'-NCR. Recombinant viral genome RNA infection models confirmed that PV1 lacking this region was non-infectious. CONCLUSION: In this study, we not only elucidated the mechanisms by which ozone induces PV1 inactivation but also determined that the 80-124 nt region in the 5'-NCR is targeted by ozone to achieve this inactivation.
Authors: Bernardí Bayarri; Alberto Cruz-Alcalde; Núria López-Vinent; María M Micó; Carme Sans Journal: J Hazard Mater Date: 2021-03-13 Impact factor: 14.224
Authors: Emanuela Sozio; Amato De Monte; Giovanni Sermann; Flavio Bassi; Davide Sacchet; Francesco Sbrana; Andrea Ripoli; Francesco Curcio; Martina Fabris; Stefania Marengo; Daniele Italiani; Daniela Luciana Boccalatte-Rosa; Carlo Tascini Journal: Int Immunopharmacol Date: 2021-06-12 Impact factor: 5.714
Authors: Emmeline L Blanchard; Justin D Lawrence; Jeffery A Noble; Minghao Xu; Taekyu Joo; Nga Lee Ng; Britney E Schmidt; Philip J Santangelo; M G Finn Journal: medRxiv Date: 2020-05-26
Authors: Alessandra Gavazza; Andrea Marchegiani; Giacomo Rossi; Marianno Franzini; Andrea Spaterna; Sara Mangiaterra; Matteo Cerquetella Journal: Front Public Health Date: 2020-08-25