Puja Debnath1, Jayant Dewangan2, Divya Tandon2, Vivek Govila1, Mona Sharma1, Virendra Kumar3, Smita Govila4, Srikanta Kumar Rath2. 1. Department of Periodontics, Babu Banarasi Das College of Dental Sciences, BBD University, Lucknow, Uttar Pradesh, India. 2. Genotoxicity Lab, Division of Toxicology & Experimental Medicine, CSIR- Central Drug Research Institute, Lucknow, Uttar Pradesh, India. 3. Department of Dentistry, Sarojini Naidu Medical College, Agra, Uttar Pradesh, India. 4. Department of Conservative Dentistry, Babu Banarasi Das College of Dental Sciences, BBD University, Lucknow, Uttar Pradesh, India.
Abstract
OBJECTIVES: Impaired plasminogen activator inhibitor-1 (PAI-1), controlling coagulation and the fibrinolytic system is supposed to be involved in the pathogenesis of periodontitis. This study was performed to examine the association of PAI-1 gene polymorphisms with Chronic Periodontitis (CP) and alveolar bone loss severity involved with the disease and for understanding the role of genetic contributions in disease progression. METHODS: 87 volunteers were included in the study. Genomic DNA was isolated from peripheral blood, subsequently, DNA samples were subjected to polymerase chain reaction and endonuclease digestion. Direct gene sequencing were performed for all the samples to identify genotype polymorphisms (rs 11560324) in the 3' untranslated region of PAI-1 gene. For bone loss assessment full mouth IOPA was taken. RESULTS: Statistical analysis showed that for SNP PAI-I in 3' UTR, genotype CC (homozygous mutant) and allele C (mutant) has a risk associated with CP, although statistically significant association was not found. An increased risk of association of disease severity with CG (heterozygous mutant) and CC (homozygous mutant) genotypes, i.e., an increased carriage rate of genotype CG and CC (homozygous mutant) was evident with the increase in the severity of CP, highlighting an increased susceptibility to CP due to this gene polymorphisms. CONCLUSION: PAI-1 genotype has a risk association with CP and alveolar bone loss severity in North-Indian population.
OBJECTIVES: Impaired plasminogen activator inhibitor-1 (PAI-1), controlling coagulation and the fibrinolytic system is supposed to be involved in the pathogenesis of periodontitis. This study was performed to examine the association of PAI-1 gene polymorphisms with Chronic Periodontitis (CP) and alveolar bone loss severity involved with the disease and for understanding the role of genetic contributions in disease progression. METHODS: 87 volunteers were included in the study. Genomic DNA was isolated from peripheral blood, subsequently, DNA samples were subjected to polymerase chain reaction and endonuclease digestion. Direct gene sequencing were performed for all the samples to identify genotype polymorphisms (rs 11560324) in the 3' untranslated region of PAI-1 gene. For bone loss assessment full mouth IOPA was taken. RESULTS: Statistical analysis showed that for SNP PAI-I in 3' UTR, genotype CC (homozygous mutant) and allele C (mutant) has a risk associated with CP, although statistically significant association was not found. An increased risk of association of disease severity with CG (heterozygous mutant) and CC (homozygous mutant) genotypes, i.e., an increased carriage rate of genotype CG and CC (homozygous mutant) was evident with the increase in the severity of CP, highlighting an increased susceptibility to CP due to this gene polymorphisms. CONCLUSION: PAI-1 genotype has a risk association with CP and alveolar bone loss severity in North-Indian population.
Authors: W C Hooper; C Lally; H Austin; M Renshaw; A Dilley; N K Wenger; D J Phillips; C Whitsett; P Rawlins; B L Evatt Journal: Thromb Res Date: 2000-08-01 Impact factor: 3.944
Authors: M L Laine; M A Farré; G González; L J van Dijk; A J Ham; E G Winkel; J B Crusius; J P Vandenbroucke; A J van Winkelhoff; A S Peña Journal: J Dent Res Date: 2001-08 Impact factor: 6.116