BACKGROUND: Sepsis is a set of serious organic manifestations caused by an infection, whose progression culminates in exacerbated inflammation and oxidative stress, poor prognosis, and high hospital costs. Antioxidants used against sepsis have been evaluated, including essential oils such as β-caryophyllene (BCP), and polyunsaturated fatty acids, such as docosahexaenoic acid (DHA). The aim of this study was to evaluate the anti-inflammatory activity of the association of these two compounds. RESULTS: Treatment with BCP-DHA, at a dose of 200 μL/animal, significantly inhibited the migration of neutrophils in a Cg-induced peritonitis model. After Staphylococcus aureus infection, in the groups treated with BCP-DHA there was a significant decrease in the total and differential count of leukocytes, increased expression of cytokines TNF-α and IFN-γ in treated groups, an increase of IL-4 and IL-5 in B/D and B/D + SA groups, and an augmentation of IL-6 and IL-12 groups in B/D + SA groups. Histological and bacterial analysis revealed lower neutrophil migration and lower bacterial load in the infected and treated groups. CONCLUSION: In general, the BCP-DHA association presented anti-inflammatory activity against two different models of acute inflammation and infection, showing promising potential as a therapeutic adjuvant in sepsis.
BACKGROUND:Sepsis is a set of serious organic manifestations caused by an infection, whose progression culminates in exacerbated inflammation and oxidative stress, poor prognosis, and high hospital costs. Antioxidants used against sepsis have been evaluated, including essential oils such as β-caryophyllene (BCP), and polyunsaturated fatty acids, such as docosahexaenoic acid (DHA). The aim of this study was to evaluate the anti-inflammatory activity of the association of these two compounds. RESULTS: Treatment with BCP-DHA, at a dose of 200 μL/animal, significantly inhibited the migration of neutrophils in a Cg-induced peritonitis model. After Staphylococcus aureus infection, in the groups treated with BCP-DHA there was a significant decrease in the total and differential count of leukocytes, increased expression of cytokines TNF-α and IFN-γ in treated groups, an increase of IL-4 and IL-5 in B/D and B/D + SA groups, and an augmentation of IL-6 and IL-12 groups in B/D + SA groups. Histological and bacterial analysis revealed lower neutrophil migration and lower bacterial load in the infected and treated groups. CONCLUSION: In general, the BCP-DHA association presented anti-inflammatory activity against two different models of acute inflammation and infection, showing promising potential as a therapeutic adjuvant in sepsis.
Authors: Andreza Larissa do Nascimento; Joyce Bezerra Guedes; Wêndeo Kennedy Costa; Bruno Oliveira de Veras; Júlio César Ribeiro de Oliveira Farias de Aguiar; Daniela Maria do Amaral Ferraz Navarro; Maria Tereza Dos Santos Correia; Thiago Henrique Napoleão; Alisson Macário de Oliveira; Márcia Vanusa da Silva Journal: Inflammopharmacology Date: 2022-09-12 Impact factor: 5.093
Authors: Byron Brook; Danny J Harbeson; Casey P Shannon; Bing Cai; Daniel He; Rym Ben-Othman; Freddy Francis; Joe Huang; Natallia Varankovich; Aaron Liu; Winnie Bao; Morten Bjerregaard-Andersen; Frederik Schaltz-Buchholzer; Lilica Sanca; Christian N Golding; Kristina Lindberg Larsen; Ofer Levy; Beate Kampmann; Rusung Tan; Adrian Charles; James L Wynn; Frank Shann; Peter Aaby; Christine S Benn; Scott J Tebbutt; Tobias R Kollmann; Nelly Amenyogbe Journal: Sci Transl Med Date: 2020-05-06 Impact factor: 17.956
Authors: Györgyi Horváth; Adrienn Horváth; Gréta Reichert; Andrea Böszörményi; Katalin Sipos; Edina Pandur Journal: BMC Complement Med Ther Date: 2021-05-22
Authors: Celeste de Jesus Pereira Franco; Oberdan Oliveira Ferreira; Jorddy Neves Cruz; Everton Luiz Pompeu Varela; Ângelo Antônio Barbosa de Moraes; Lidiane Diniz do Nascimento; Márcia Moraes Cascaes; Antônio Pedro da Silva Souza Filho; Rafael Rodrigues Lima; Sandro Percário; Mozaniel Santana de Oliveira; Eloisa Helena de Aguiar Andrade Journal: Molecules Date: 2022-07-22 Impact factor: 4.927