Karine Moussa1, Purnima Gurung1, Beverley Adams-Huet2, Sridevi Devaraj3, Ishwarlal Jialal4. 1. California Northstate University College of Medicine, United States of America. 2. University of Texas Southwestern Medical Center, Dallas, TX, United States of America. 3. Baylor College of Medicine, Houston, TX, United States of America. 4. California Northstate University College of Medicine, United States of America; VA Medical Center, Mather, CA, United States of America. Electronic address: Ishwarlal.jialal@cnsu.edu.
Abstract
AIMS: Metabolic Syndrome (MetS) is a common global disorder that predisposes to both Type 2 diabetes mellitus (T2DM) and cardiovascular disease (ASCVD). Adipose tissue (AT) contributes significantly to increased inflammation and insulin resistance (IR) in MetS which appear to be the crucial underpinnings of MetS. Compared to macrophages and lymphocytes in human subcutaneous AT (SAT), there is sparse data on the role of other immune cells, especially eosinophils (EOS). In this study, we investigated the abundance of EOS in the SAT of 19 patients with MetS without diabetes, ASCVD, smoking or any inflammatory condition, and matched controls. METHODS: SAT EOS were quantified by immunohistochemistry. RESULTS: Both circulating and SAT EOS were significantly increased 2-fold in MetS and correlated with each other. Circulating EOS correlated significantly with triglycerides (TG), high-sensitivity CRP, leptin, and IL-6. SAT EOS correlated significantly with plasma glucose, TG, FFA, adipose-IR, leptin, IL-6, endotoxin, chemerin and inversely with adiponectin. They also correlated with SAT markers of fibrosis: collagen and Sirius red staining of SAT. CONCLUSION: We make the novel and seminal observation that eosinophils are increased in SAT of MetS patients, and are associated with the pro-inflammatory state. Hence, in humans, they appear to contribute to the dysregulation of SAT biology in MetS. Published by Elsevier Inc.
AIMS: Metabolic Syndrome (MetS) is a common global disorder that predisposes to both Type 2 diabetes mellitus (T2DM) and cardiovascular disease (ASCVD). Adipose tissue (AT) contributes significantly to increased inflammation and insulin resistance (IR) in MetS which appear to be the crucial underpinnings of MetS. Compared to macrophages and lymphocytes in human subcutaneous AT (SAT), there is sparse data on the role of other immune cells, especially eosinophils (EOS). In this study, we investigated the abundance of EOS in the SAT of 19 patients with MetS without diabetes, ASCVD, smoking or any inflammatory condition, and matched controls. METHODS:SAT EOS were quantified by immunohistochemistry. RESULTS: Both circulating and SAT EOS were significantly increased 2-fold in MetS and correlated with each other. Circulating EOS correlated significantly with triglycerides (TG), high-sensitivity CRP, leptin, and IL-6. SAT EOS correlated significantly with plasma glucose, TG, FFA, adipose-IR, leptin, IL-6, endotoxin, chemerin and inversely with adiponectin. They also correlated with SAT markers of fibrosis: collagen and Sirius red staining of SAT. CONCLUSION: We make the novel and seminal observation that eosinophils are increased in SAT of MetS patients, and are associated with the pro-inflammatory state. Hence, in humans, they appear to contribute to the dysregulation of SAT biology in MetS. Published by Elsevier Inc.
Authors: Bárbara M Oliveira; Ana Pinto; Alexandra Correia; Paula G Ferreira; Manuel Vilanova; Luzia Teixeira Journal: Sci Rep Date: 2020-02-04 Impact factor: 4.379
Authors: Natália R T Amorim; Glaucia Souza-Almeida; Tatiana Luna-Gomes; Patricia T Bozza; Claudio Canetti; Bruno L Diaz; Clarissa M Maya-Monteiro; Christianne Bandeira-Melo Journal: Front Endocrinol (Lausanne) Date: 2020-09-29 Impact factor: 5.555