| Literature DB >> 31201852 |
Serafeim C Chaintoutis1, Elpida Papadopoulou2, Angeliki Melidou2, Anna Papa2, Chrysostomos I Dovas3.
Abstract
Lineage 2 West Nile virus (WNV) strains have been implicated in severe encephalitis outbreaks in humans and equines residing in Europe. WNV molecular characterization is important for the development of diagnostic assays, as well as for obtaining molecular information, which is necessary for epidemiological investigations of virus transmission in areas at risk. For whole genome sequencing of lineage 2 WNV strains, directly from biological specimens, a PCR-based NGS protocol was developed. The method was applied in WNV-positive specimens obtained from animal, human and mosquito hosts in Greece. The results of its application indicate that, even in cases of low virus titers, the developed PCR-based NGS approach is able to provide whole genome sequences of lineage 2 WNV strains.Entities:
Keywords: Lineage 2; Next generation sequencing; PCR-Based; West Nile virus; Whole genome sequencing
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Year: 2019 PMID： 31201852 DOI： 10.1016/j.mcp.2019.06.002
Source DB: PubMed Journal: Mol Cell Probes ISSN： 0890-8508 Impact factor: 2.365