Jennifer Chang1, Steven B Blanchard2, L Jack Windsor3, Richard L Gregory3, Yusuke Hamada4. 1. Department of Periodontics and Dental Hygiene, University of Texas Health Science Center at Houston School of Dentistry, 7500 Cambridge Street, Houston, TX, 77045, USA. 2. Department of Periodontics and Allied Dental Programs, Indiana University School of Dentistry, Indiana, USA. 3. Department of Biomedical and Applied Science, Indiana University School of Dentistry, 1121 W Michigan Street, Indianapolis, IN, 46202, USA. 4. Department of Periodontics and Allied Dental Programs, Indiana University School of Dentistry, Indiana, USA. yuhamada@iupui.edu.
Abstract
OBJECTIVES: This study aimed to (1) compare the amounts of growth factors from platelet-rich fibrin (PRF) between chronic periodontitis and periodontally healthy subjects and (2) evaluate the relationships between the amounts of growth factors from PRF with complete blood counts (white blood cell (WBC) and platelet counts) and the serum concentrations of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α). MATERIALS AND METHODS: Venous blood was collected from chronic periodontitis (test) and periodontally healthy subjects (control). PRF and serum were collected from the centrifuged blood. Liquid exudates from the compression of PRF were collected. The compressed PRF membranes were incubated in saline, and eluted aliquots were collected at 1, 24, and 72 h, and the membranes were then digested with trypsin. Epidermal growth factor, insulin-like growth factor-1, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor in the exudates and eluents were quantified by ELISA. Serum was used for IL-1β, IL-6, and TNF-α quantifications. Complete blood counts were measured. RESULTS: There were no significant differences in the amounts of growth factors from PRF exudates and membranes measured between groups (all p > 0.05). The test group had significantly higher WBC (p < 0.05). However, there was no significant correlation between the WBC and the amounts of the growth factors from PRF (all p > 0.05). CONCLUSIONS: PRF can be utilized as an autologous source of growth factors not affected by periodontal condition and WBC level. CLINICAL RELEVANCE: The amounts of growth factors from PRF were not affected by the periodontal condition of the patient.
OBJECTIVES: This study aimed to (1) compare the amounts of growth factors from platelet-rich fibrin (PRF) between chronic periodontitis and periodontally healthy subjects and (2) evaluate the relationships between the amounts of growth factors from PRF with complete blood counts (white blood cell (WBC) and platelet counts) and the serum concentrations of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α). MATERIALS AND METHODS: Venous blood was collected from chronic periodontitis (test) and periodontally healthy subjects (control). PRF and serum were collected from the centrifuged blood. Liquid exudates from the compression of PRF were collected. The compressed PRF membranes were incubated in saline, and eluted aliquots were collected at 1, 24, and 72 h, and the membranes were then digested with trypsin. Epidermal growth factor, insulin-like growth factor-1, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor in the exudates and eluents were quantified by ELISA. Serum was used for IL-1β, IL-6, and TNF-α quantifications. Complete blood counts were measured. RESULTS: There were no significant differences in the amounts of growth factors from PRF exudates and membranes measured between groups (all p > 0.05). The test group had significantly higher WBC (p < 0.05). However, there was no significant correlation between the WBC and the amounts of the growth factors from PRF (all p > 0.05). CONCLUSIONS: PRF can be utilized as an autologous source of growth factors not affected by periodontal condition and WBC level. CLINICAL RELEVANCE: The amounts of growth factors from PRF were not affected by the periodontal condition of the patient.
Authors: David M Dohan; Joseph Choukroun; Antoine Diss; Steve L Dohan; Anthony J J Dohan; Jaafar Mouhyi; Bruno Gogly Journal: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Date: 2006-01-10
Authors: Amarpreet Sabharwal; Isaac S Gomes-Filho; Elizabeth Stellrecht; Frank A Scannapieco Journal: Periodontol 2000 Date: 2018-10 Impact factor: 7.589