Literature DB >> 3119695

A modified procedure for the preparation of insoluble chromogenic substrates for the determination of proteolytic activity.

I Safarík1.   

Abstract

New insoluble chromogenic substrates for the determination of proteolytic activity were prepared by cross-linking gelatin with glutaraldehyde in the presence of congo red or nigrosin. The prepared substrates could detect approximately 0.4 microgram of trypsin per ml. The spontaneous leakage of dyes in water solutions was negligible.

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Year:  1987        PMID: 3119695     DOI: 10.1016/0165-022x(87)90030-3

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  1 in total

1.  Cis-2-dodecenoic acid signal modulates virulence of Pseudomonas aeruginosa through interference with quorum sensing systems and T3SS.

Authors:  Yinyue Deng; Calvin Boon; Shaohua Chen; Amy Lim; Lian-Hui Zhang
Journal:  BMC Microbiol       Date:  2013-10-18       Impact factor: 3.605

  1 in total

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