Literature DB >> 31193989

Data on MECOM rearrangement-driven chromosomal aberrations in myeloid malignancies.

Zhenya Tang¹, Guilin Tang¹, Shimin Hu¹, Keyur P Patel¹, C Cameron Yin¹, Wei Wang¹, Pei Lin¹, Gokce A Toruner¹, Chi Y Ok¹, Jun Gu², Xinyan Lu³, Joseph D Khoury¹, L Jeffrey Medeiros¹.

Abstract

Data in this article presents the results of conventional cytogenetics and fluorescence in situ hybridization (FISH) analyses in 129 patients with confirmed MECOM rearrangement (https://doi.org/10.1016/j.cancergen.2019.03.002) [1]. Generally, the MECOM rearrangement has arisen through translocation, inversion, and insertion and/or unknown mechanism. In addition to the typical chromosomal aberrations, inv(3)(q21q26.2) and t(3; 3)(q21; q26.6) [2-4], over 50% of cases presented here exhibit a wide spectrum of MECOM rearrangement-driven, atypical chromosomal aberrations, including inv(3) with breakpoint other than 3q21; t(1; 3); t(2; 3); t(3; 6); t(3; 8); t(3; 12); t(3; 17); t(3; 21) as well as an insertion of 3q26.2 into different chromosomes. These cases are thoroughly characterized by karyotyping, interphase-, metaphase-, map-back FISH and whole chromosomal painting (WCP) analyses.

Entities: Disease Gene Species

Keywords: Fluorescence in situ hybridization (FISH); Insertion; Inversion; MECOM rearrangement; Translocation

Year: 2019 PMID： 31193989 PMCID： PMC6545385 DOI： 10.1016/j.dib.2019.104025

Source DB: PubMed Journal: Data Brief ISSN： 2352-3409

Specifications Table This dataset provides so far the largest number of cases from a single institute about MECOM rearrangement in myeloid malignancies [1]. Scientists and/or pathologists in the field of cytogenetic and genomic diagnostics can use these cases as references for complex cases involving chromosome 3 abnormalities. The MECOM rearrangement has been confirmed by FISH analysis in all cases, comparing to cases with chromosome 3 abnormalities but without further confirmation of a MECOM rearrangement in previous studies [2], [3], [4], [5], [6]. The majority of cases with atypical chromosomal abnormalities have been thoroughly analyzed by interphase-, metaphase-, map-back FISH and/or WCP. Therefore, the results, especially the ISCN description of all chromosomal aberrations, are accurate and reliable.

Data

This is an intensive characterization of complexities of chromosomal aberrations involving 3q26.2/MECOM rearrangement in 129 cases with a variety of myeloid malignancies. The majority of cases with atypical and/or complex karyotype has been thoroughly analyzed by interphase-, metaphase-, map-back FISH, and/or whole chromosome painting (WCP). In addition to the results of conventional cytogenetics and FISH analyses, other general clinical information including age, gender, pathologic diagnosis and outcomes, are also included (Table 1). We believe that all these cases can be practically useful for scientists and/or pathologists in the field of cytogenetic and genomic diagnostics. They can be used as references for all newly encountered case with a putative 3q26.2 abnormality/MECOM rearrangement.

Table 1

General information, karyotyping and MECOM FISH results in 129 cases of myeloid malignancies.

Case#	Sex	Age (y)	Diagnosis	D/A	FU (m)	Karyotype results	FISH results
1	M	70	AML	D	2	45,XY,inv(3)(p23q26.2),-7,add(8)(q24.1),del(20)(q11.2q13.1)[20]	ish inv(3)(p23q26.2)(3′MECOM+,5′MECOM+)[3].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[153/200]
2	F	77	AML	D	1	43,X,-X,inv(3)(q13.3q26.2),del(5)(q22q35),add(6)(p21.1),-7,-17,-19,+mar[8]/43,idem,del(11)(p11.2)[3]/44,idem,+mar[3]/44,idem,i(5)(q10),+mar[2]/46,XX[4]	ish inv(3)(q13.3)(3′MECOM+)(q26.2)(5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[172/200]
3	M	67	AML	D	10	45,XY,inv(3)(q21q26.2),-7[3].	ish inv(3)(q21)(5′MECOM+)(q26.2)(5′MECOM+,3′MECOM+)[3].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[164/200]
4	M	72	AML	D	13	46,XY,inv(3)(q21q26.2)[20]	ish inv(3)(q21)(5′MECOM+)(q26.2)(3′MECOM+)[1].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[76/200]
5	M	77	MDS, AML	D	4	46,XY,inv(3)(q21q26.2)[20]	ish inv(3)(q21)(5′MECOM+)(q26.2)(3′MECOM+)[1].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[42/200]
6	M	74	MDS	D	32	46,XY,inv(3)(q21q26.2)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[127/200]
7	M	55	AML	D	6	46,XY,inv(3)(q21q26.2)[19]/46,XY[1]	ish inv(3)(q21)(5′MECOM+)(q26.2)(3′MECOM+)[1].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[158/200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[37/200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[39/200]
8	F	63	MDS	D	10	46,XX,inv(3)(q21q26.2)[20]	ish inv(3)(q21)(5′MECOM+)(q26.2)(3′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[114/200]
9	M	68	AML/MDS	D	2	45,X,-Y[15]/45,idem,inv(3)(q21q26.2)[5]	ish inv(3)(q21; q26)(3′MECOM+,5′MECOM+)[1].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[24/200]
10	F	66	AML	D	1	46,XX,inv(3)(q21q26.2)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[109/200]
11	M	41	AML	D	4	46,XY,inv(3)(q21q26.2)[15]/47,idem,+mar[2]/46,XY[3]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[36/200]
12	M	22	AML	D	4	45,XY,inv(3)(q21q26.2),-7[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[110/200]
13	F	29	t-MDS	A, CR (SCT)	37	46,XX,inv(3)(q21q26.2)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[76/200]
14	M	74	AML/MDS	D	0	46,XY,inv(3)(q21q26.2)[20]	ish inv(3)(q21)(5′MECOM+)(q26.2)(3′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[100/200]
15	M	72	MDS	D	0	45,XY,inv(3)(q21q26.2),del(5)(q31q35),-7,del(12)(p11.2p13)[16]/45,idem,del(11) (p11.2)[2]/46,XY[2]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[102/200]
16	M	46	AML	D	5	46,XY,inv(3)(q21q26.2)	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[146/200]
17	F	68	MDS, AML	D	3	44,XX,-3,inv(3)(q21q26.2),del(5)(q13q33),der(6)t(3; 6)(q23; p23),add(7)(q22),del(12)(p11.2p13),-20[17]/45,XX,inv(3)(q21q26.2),del(5)(q13q33),add(7)(q22),del(12)(p11.2p13),-20[3]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[138/200]
18	F	72	AML	D	3	46,XX,inv(3)(q21q26.2)[4]/46,idem,der(16)t(1; 16)(q21; q13)[15]/46,idem,del(X)(q21)[1]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[138/200]
19	M	32	AML	A, CR	21	46,XY,inv(3)(q21q26.2)[16]/46,XY[4]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[17/201
20	F	27	AML	D	4	43∼45,XX,add(2)(q32),add(3)(q27),inv(3)(q21q26.2),del(5)(q22q35),-7,del(14)(q22)[cp20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[174/200]
21	F	49	AML	D	0	46,XX,inv(3)(q21q26.2)[2]/46,idem,der(X)del(X)(p22.1p22.3)add(X)(q24)[9]/45,idem,-7[9]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[32/200]
22	F	77	MDS	A, PR	3	46,XX,inv(3)(q21q26.2),r(7)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[85/200]
23	M	77	AML	A, PR	17	47,XY,inv(3)(q21q26.2),del(7)(q22q34),+21[20]	nucish(3′MECOMx3,5′MECOMx2)(3′MECOM con 5′MECOMx2)[54/200]
24	M	91	MDS	D	1	47,XY,inv(3)(q21q26.2),+8[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[90/200]
25	F	57	AML	D	0	46,XX,inv(3)(q21q26.2)[15]/46,idem,add(2)(q11.2)[4]/46,idem,der(2)t(2; 11)(q11.2; q13)[1]	ish inv(3)(q21)(3′MECOM+)(q26.2)(5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[145/200]
26	M	80	MDS	D	1	45,XY,inv(3)(q21q26.2),-7[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[131/200]
27	F	73	polycythemia vera with MF-3	D	0	46,XX,t(12; 18)(q13; q21.1)[4]/46,idem,add(13)(q12)[6]/46,idem,inv(3)(q21q26.2)[4]/46,idem,del(13)(q12q22)[3]/46,idem,add(6)(p21)[2]/45,idem,dic(6; 11)(p21; p11.2),add(7)(p22)[1]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[24/200]/(MECOMx4)[17/200]
28	F	39	AML	A, PR (SCT)	11	46,XX,inv(3)(q21q26.2)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[140/200]
29	M	48	AML	A, CR (SCT)	7	45,XY,inv(3)(q21q26.2),-7[19]/46,XY[1]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[117/200]
30	M	17	AML	A, NR	7	45,XY,inv(3)(q21q26.2),-7[18]/45,idem,+2mar[cp2]	ish inv(3)(q21)(3′MECOM+)(q26.2)(5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[146/200]
31	M	78	AML	D	4	45,XY,inv(3)(q21q26.2),-7,inv(9)(p12q13)[9]/46,XY,inv(9)(p12q13)[11]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[94/200]
32	F	70	MDS	D	1	46,XX,inv(3)(q21q26.2)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[103/200]
33	M	52	AML	A, PR	4	46,XY,inv(3)(q21q26.2),t(7; 9)(p15; p23)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[124/200]
34	M	67	AML	D	2	45,X,-Y,inv(3)(q21q26.2)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[147/200]
35	M	65	t-MDS; metastatic PROSTATIC ADENOCARCINOMA	A, PR	2	42∼49,Y,add(X)(p22),inv(3)(q21q26.2),-4,-5,del(5)(q31q35),-7,-8,-9,add(11)(p15),-12,add(12)(p13),+14,+15,-16,del(17)(p12),-19,-21,+2∼8 mar[cp20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[15/200]
36	F	45	AML	A, PR	1	46,XX,inv(3)(q21q26.2)[14]/46,XX[6]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[68/200]
37	F	53	AML	A, NR	0	45,XX,inv(3)(q21q26.2),-7[19]/46,idem,dup(1)(q25q32),-14,-16,+3mar[1]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[135/200]
38	M	63	CML, BP	A, PR	11	46,XY,t(9; 22)(q34; q11.2)[5]/46,idem,inv(3)(q21q26.2)[10]/46,idem,inv(16)(p13.1q22)[5]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[43/200]
39	F	45	CML, CP	D	5	46,XX,inv(3)(q21q26.2),t(9; 22)(q34; q11.2)[4]/46,idem,add(4)(q22),+12,-17,-17,+21[1]/46,XX[5]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[126/200]
40	M	61	CML, BP/AML	D	12	46,XY,inv(3)(q21q26.2),t(6; 13)(q13; q34),t(9; 22)(q34; q11.2),t(16; 17)(q13; q25)[19]/46,XY[1]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[104/200]
41	F	46	CML, BP	A, PR	1	45,XX,inv(3)(q21q26.2),-7,t(9; 22)(q34; q11.2),-15,+mar[10]/46,idem,+mar[7]/46,idem,+8[1]/45,XX,t(1; 2)(p34; p25),inv(3)(q21q26.2),-7,t(9; 22)[2]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[115/200]
42	M	37	CML, CP	A, PR	8	46,XY,inv(3)(q21q26.2),t(9; 22)(q34; q11.2)[17]/46,XY[1]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[171/200]
43	M	41	t-AML	D	9	46,XY,inv(3)(q21q26.2)[3]/45,idem,-7[16]/46,XY[1]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[53/200]nucish(MECOMx2)[200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[15/200]
44	F	56	AML/MDS	D	5	46,XX,inv(3)(q21q26.2)x2[1]/45,XX,idem,-7[14]/46,XX[5]	ish inv(3)(q21)(5′MECOM+)(q26.2)(3′MECOM+)x2[3].nucish(MECOMx2)(3′MECOM sep 5′MECOMx2)[85/200]
45	F	70	AML	D	4	46,XX,inv(3)(q21q26)x2[16]/46,idem,+1,der(1; 15)(q10; q10)[4]	ish inv(3)(q21)(3′MECOM+)(q26.2)(5′MECOM+)x2[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx2)[40/200]
46	M	44	MDS, AML	A, PR	3	43∼45,XY,inv(3)(p21q28),inv(3)(q21q26.2),del(5)(q22q35),-7,-12,der(12; 14)(q10; q10),-14,-17,del(17)(p12),+1∼3mar[cp20]	ish inv(3)(q21)(3′MECOM+)(q26.2)(5′MECOM+)[2]/inv(3)(p21q28)(MECOM+)[1].nuc ish(MECOMx2)(3′MECOM sep 5′MECOMx1)[44/200]
47	F	46	CML, BP	A, PR	5	46,XX,add(2)(q33),inv(3)(q26.2q28),t(9; 22)(q34; q11.2)[4]/47,idem,+8[8]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[51/200]
48	F	83	MDS	D	2	44,XX,der(3)del(3)(p25)inv(3)(q21q26.2)add(3)(q26.2),-5,-7,-12,add(17)(p11.2),i(22)(q10),+r[6]/43,XX,der(3)inv(3)(q21q26.2)add(3)(q26.2),-5,-7,-15,add(17)(p11.2),i(22)(q10),+mar[6]/42∼45,XX,der(3)inv(3)(q21q26.2)add(3)(q26.2),-5,-7,-12,add(17)(p11.2),-20,i(22)(q10),+mar[cp6]/46,XX[2]	ish der(3)inv(3)(q21)(3′MECOM-)(q26.2)(5′MECOM+)add(3)(q26.2)[5].nucish(3′MECOMx1,5′MECOMx2)(3′MECOM con 5′MECOMx1)[86/200]
49	M	65	MDS	D	11	45,XY,der(3)ins(3; ?)(q21; ?)inv(3)(q21q26.2),der(5)del(5)(q13q33)t(5; 11)(q35; q22),-7,der(11)t(5; 11),inv(12)(p12q21),add(13)(q34),-15,del(20)(q11.2q13.3),+mar,+2∼5dmin[17]/46,XY[2]	ish der(3)ins(3; ?)(q21; ?)inv(3)(q21)(5′MECOM+)q26.2)(3′MECOM+)[2].nuc ish(MECOMx2)(3′MECOM sep 5′MECOMx1)[21/200]
50	F	57	AML	D	11	46,XX,der(3)t(3; 11)(q13.2; q21),der(11)t(3; 11)inv(3)(q21q26.2),del(12)(p13)[18]/46,idem[cp2]	ish der(3)t(3; 11)(q13.2; q21)(MECOM-),der(11)t(3; 11)inv(3)(q21)(3′MECOM+)(q26.2)(5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[145/200]. MLL FISH negative
51	M	69	AML	D	4	46,XY,der(3)t(3; 14)(p13; q32)inv(3)(q21q26.2),der(14)t(3; 14)[18]/46,idem,del(7)(p12)[2]	ish der(3)t(3; 14)(p13; q32)inv(3)(q21)(5′MECOM+)(q26.2)(3′MECOM+)[4].nuc ish(MECOMx2)(3′MECOM sep 5′MECOMx1)[132/200]
52	M	56	MDS	D	8	46,XY,-7,+mar[1]/46,sl,der(3)t(3; 3)(p21; q13),der(3)t(3; 3)inv(3)(q21q26.2)[9]/47,sdl,+mar[7]/47∼48, sl,+1∼2mar[cp3]	ish der(3)t(3;3)(p21; q13)(MECOM-),der(3)t(3;3)inv(3)(q21q26.2)(3′MECOM+,5′MECOM+,MECOM+)[2].nuc ish(MECOMx2)(3′MECOM sep 5′MECOMx1)[107/200]
53	F	45	AML/MDS	A, CR (SCT)	55	46,XX,t(1; 3)(q32; q26.2)[20]	ish t(1; 3)(q32; q26)(5′MECOM+; 3′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[180/200]nucish(MECOMX2)[200]
54	F	65	donor cell AML	A, PR	1	//46,XY,der(2)t(2; 3)(q21; q26.2),der(3; 3)(q10; q10)add(3)(q26.2)t(2; 3),del(20)(q11.2q13.3)[12]	ish der(2)t(2; 3)(p21; q26.2)(5′MECOM+),der(3; 3)(q10; q10)add(3)(q26.2)(3′MECOM+)t(2; 3)(3′MECOM+)[1].nuc ish(3′MECOMx3,5′MECOMx2)(3′MECOM con 5′MECOMx1)[176/200]/(MECOMx2)(3′MECOM sep 5′MECOMx1)[24/200]
55	F	77	MDS + CML	D	1	47,XX,t(2; 3)(p16; q26.2),del(7)(q22q34),t(9; 22)(q34; q11.2),+21[20]	ish t(2; 3)(p16; q26.2)(5′MECOM+; 3′MECOM+)[3].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[191/200]
56	F	46	MDS/AML	D	23	46,XX,t(2; 3)(p21; q26.2)[20]	ish t(2; 3)(5′MECOM+; 3′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[185/200]
57	F	69	MDS/CMML	D	12	46,XX,t(2; 3)(p21; q26.2)[20]	ish t(2; 3)(p21; q26.2)(5′MECOM+; 3′MECOM+)[5].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[175/200]
58	F	80	MDS	D	4	45,XX,t(2; 3)(p21; q26.2),-7[2]/46,t(2; 3),r(7)(p21q11.2)[2]/46,XX[16]	ish t(2; 3)(p21; q26.2)(5′MECOM+; MECOM+)[1].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[35/200]
59	F	56	CML, BP	D	8	45,XX,t(2; 3)(p21; q26.2),-7,t(9; 22)(q34; q11.2)[19]/45,idem,add(6)(q21)[1]	ish t(2; 3)(p21; q26.2)(5′MECOM+; 3′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[190/200]
60	M	65	MDS/AML	D	13	46,XY,t(2; 3)(p23; q26.2)[20]	ish t(2; 3)(5′MECOM+; 3′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[194/200]
61	M	63	AML	D	1	46,XY,t(2; 3)(p23; q26.2),r(7)[20]	ish t(2; 3)(p23; q26.2)(5′MECOM+; MECOM+)[2].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[172/200]
62	M	53	AML	D	11	46,XY,t(2; 3)(p23; q26.2)[19]/47,idem,+21[1	nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[158/200]
63	F	38	MDS	D	6	46,XX,t(2; 3)(p23; q26.2)[5]/46,idem,-5,+r[13]/46,XX[2]	nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[172/200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[123/200]
64	F	57	AML	A, PR (SCT)	8	46,XX,t(2; 3)(p23; q26.2),del(5)(q22q35)[20]	ish t(2; 3)(p23; q26.2)(5′MECOM+; MECOM+)[2].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[197/200]
65	M	39	CML, CP	A, PR	5	47,XY,+8[4]/46,X,-Y,+8[12]/46,XY,t(2; 3)(p23; q26.2),t(9; 22)(q34; q11.2)[4]	ish t(2; 3)(p23; q26.2)(5′MECOM+; MECOM+)[1].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[11/200]
66	F	51	MDS	D	22	46,XX,t(2; 3)(p23; q26.2)[17]/47,idem,+mar[2]/46,idem,der(18)t(1; 18)(q21; p11.2)[1]	nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[70/200]ish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[38/200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[95/200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[180/200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[178/200]
67	M	52	AML	A, NR	5	45,XY,der(3)t(3; 3)(q21; q26.2),der(3)t(5; 17)(q22; p12)t(3; 5)(p12; q13)t(3; 3),t(4; 18)(p16; q21.1),-5,der(7)del(7)(p12)del(7)(q11.2),der(17)t(3; 7)(p22; q22)t(7; 17)(q32; p11.2),-20,+mar[20]	ish der(3)t(3; 3)(q21; q26.2)(5′MECOM+), der(3)t(5; 17)(q22; p12)t(3; 5)(p12; q13)t(3; 3)(MECOM+,MECOM+)[2].nuc ish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[200]
68	M	73	AML	D	3	46,XY,t(3; 3)(q21; q26.2)[11]/45,idem,del(6)(q21),-7[7]/46,idem,del(7)(q22)[1]/45,idem,del(16)(p11.1)[1]	ish t(3; 3)(q21; q26.2)(3′MECOM+; MECOM+,5′MECOM+)[4].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[190/200]
69	F	73	MDS	D	5	46,XX,del(5)(q13q33)[2]/46,idem,t(3; 3)(q21; q26.2),del(7)(q22q35)[18]	ish t(3; 3)(q21; q26.2)(5′MECOM+; MECOM+,3′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[174/200]
70	F	74	AML	D	8	44∼45,XX,t(3; 3)(q21; q26.2),-7[cp3]/46,XX[17].	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[36/200]
71	M	18	AML	A, NR	7	45,XY,t(3; 3)(q21; q26.2),-7[18]/46,XY[2]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[36/200]
72	M	65	AML	A, NR	4	43,XY,der(1)inv(1)(p22q32)add(1)(q32),t(3; 3)(q21; q26.2),del(5)(q31q35),-7,del(12)(p11.2),-14,-16,add(19)(q13.3),add(20)(q11.2)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[74/200]
73	M	40	AML	A, NR	0	45,XY,t(3; 3)(q21; q26.2),-7[8]/45,idem,+1,der(1; 15)(q10; q10),del(14)(q11.2q24)[2]/46,XY[1]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[62/200]
74	M	58	AML	D	20	46,XY,t(3; 3)(q21; q26.2)[3]//46,XX[5]	ish t(3; 3)(3′MECOM+; MECOM+)[1]/nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[40/200]
75	F	40	AML	D	0	47,XX,t(3; 3)(q26.2; q21),t(9; 22)(q34; q11.2),del(11)(p11.2p12),+19[11]/47,idem,del(8)(q12q23)[1]/47,XX,t(3; 3),del(8)(q12q23),t(9; 22)(q34; q11.2),del(11)(p12p15),der(19)t(19; 22)(p13.1; q11.2)t(9; 22),+19[4]/47∼48,XX,t(3; 3),der(7)t(7; 22)(q11.2; p11.2),der(9)t(9; 22),+19,der(22)t(7; 22)t(9; 22),+der(22)t(9; 22),+1∼2mar[cp5]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[193/200]
76	M	19	AML	D	12	46,XY,t(3; 6)(q26.2; p23),der(4)t(3; 4)(q12; p16),del(5)(q13q33),add(18)(q23)[3]/46,XY,t(3; 6)(q26.2; p23),der(4)t(3; 4)(q12; p16),add(5)(q21)[1]/46,XY,t(3; 6)(q26.2; p23),der(4)t(3; 4)(q12; p16),add(5)(q21),der(7)t(3; 7)(q26.2; q36)[1]/46,XY[20]	ish t(3; 6)(q26.2; p23)(3′MECOM+; 5′MECOM+),der(4)t(3; 4)(q12; p16)(MECOM+),der(7)t(3; 7)(q26.2; q36)(MECOM+)[1].nuc ish(3′MECOMx3,5′MECOMx4)(3′MECOM con 5′MECOMx3)[11/200]
77	F	64	AML	A, NR	0	46,XX,t(3; 6)(q26.2; q25)[20]	ish t(3; 6)(q26.2; q25)(3′MECOM+; 5′MECOM+)[2].nucish (MECOMx2)(3′MECOM sep 5′MECOMx1)[193/200]
78	M	67	MDS	D	22	46,XY,t(3; 6)(q26.2; q26)[23]	ish t(3; 6)(q26.2; q26)(3′MECOM+; 5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[195/200]
79	M	70	AML	D	2	46,XY,t(3; 6)(q26.2; q26)[20]	ish t(3; 6)(q26.2; q26)(3′MECOM+; 5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[190/200]
80	M	66	AML	D	2	46,XY,t(3; 8)(q26.2; q24.1)[5]/45,idem,-7[10]/43∼45,idem,dup(1)(q12q25),-7,-13[cp5]	ish t(3; 8)(q26.2; q24.1)(3′MECOM+; 5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[126/200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[16/200] (AB-14-24500)
81	F	75	MDS	A, NR	7	46,XX,t(3; 8)(q26.2; q24.1)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[186/200]
82	M	21	MDS, Fanconi's anemia	D	8	46,XY,t(3; 8)(q26.2; q24.1),der(7)t(7; 8)(q22; q13)t(3; 8),der(22)t(1; 22)(q25; q13)[6]/46,idem,der(2)t(2; 21)(q37; q11.2)[11]/46,idem,del(2)(q31)[3]	ish t(3; 8)(q26.2; q24.1)(3′MECOM+; 5′MECOM+),der(7)t(7; 8)(q22; q13)t(3; 8)(5′MECOM+)[2].nuc ish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx1)[184]
83	M	63	AML	D	0	46,XY,t(3; 8)(q26.2; q24.2)[16]/46,XY[4]	ish t(3; 8)(q26.2; q24.2)(3′MECOM+; 5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[188/200]
84	M	59	AML	D	3	46,XY,t(3; 8)(q26.2; q24.2)[20]	ish t(3; 8)(q26.2; q24.2)(3′MECOM+; 5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[166/200]
85	F	65	AML	D	1	45,XX,t(3; 8)(q26.2; q24),-7[17]/46,idem,+21[1]/47,idem,+21,+22[2]	ish t(3; 8)(3′MECOM+; 5′MECOM+)[3].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[195/200]
86	M	48	t-AML	D	0	46,XY,t(3; 10; 21)(q26.2; p14; q22)[20]	ish t(3; 10; 21)(q26.2; p14; q22)(MECOM+; 5′MECOM+; MECOM-)[3].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[166/200].isht(3; 10; 21)(q26.2; p14; q22)(wcp21+; wcp21-;wcp21+)[3]
87	M	61	AML	D	12	45,XY,t(3; 12)(q26.2; p13),-7[2]/46,XY,idem,+mar[10]/45,idem,-5,add(8)(p23),+mar[7]	ish t(3; 12)(q26.2; p13)(3′MECOM+; 5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[192/200]
88	M	45	AML	A, CR (SCT)	70	46,XY,t(3; 12)(q26.2; p13)[10]/46,XY[10]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[54/200]
89	F	45	AML	A, PR (SCT)	36	46,XX,t(3; 12)(q26.2; p13)[19]/46,XX[1]	ish t(3; 12)(q26.2; p13)(3′MECOM+; 5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[176/200]nucish(MECOMx2)[200] (AB-15-11961)
90	F	33	AML	D	5	45,XX,t(3; 12)(q26.2; p13),-7[20]	ish t(3; 12)(q26.1; p13)(3′MECOM+; 5′MECOM+)[1].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[200]
91	F	63	MDS	A, PR (SCT )	16	46,XX,t(3; 12)(q26.2; p13)[2]/46,XX[23]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[131/200]
92	F	60	AML	D	2	46,XX,t(3; 12)(q26.2; p13)[3]/41∼45,idem,-22[cp6]	ish t(3; 12)(q26.2; p13)(5′ETV6+; 3′ETV6+)[2].nucish(ETV6x2)(5′ETV6 sep 3′ETV6x1)[120/200].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[106/200]
93	F	38	AML	A, NR	4	46,XX,t(3; 12)(q26.2; p13),del(7)(q22q36)[18]/46,idem[cp2]	ish t(3; 12)(q26.2; p13)(MECOM+; 5′MECOM+)[2].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[188/200]
94	F	75	t-AML	A, PR	2	45,XX,t(3; 12)(q26.2; p13),-7,idic(22)(p11.2)[16]/46,XX[4]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[107/200]
95	M	59	CML, BP	D	12	49,XY,t(3; 12)(q26.2; p13),+8,+8,+i(8)(q10),i(8)(q10)x2,t(9; 22)(q34; q11.2)[19]/46,XY	ish t(3; 12)(q26; p13)(5′ETV6+; 3′ETV6+,5′ETV6-)[3].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[104/200]
96	M	28	AML	A, PR	0	45,XY,t(3; 12)(q26.2; p13),del(5)(q22q33),-7[20]	ish t(3; 12)(q26.2; p13)(3′MECOM+; 5′MECOM+)[1].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[82/200]
97	M	32	CML, BP	D	6	46,XY,t(3; 17)(q26.2; q22),t(9; 22)(q34; q11.2)[20]	ish t(3; 17)(3′MECOM+; 5′MECOM+)[4].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[132/200]
98	F	44	CML, BP	D	4	46,XX,t(3; 17)(q26.2; q22),t(9; 22)(q34; q11.2)[19]/47,idem,+der(22)t(9; 22)[1]	ish t(3; 17)(q26.2; q22)(MECOM+; 5′MECOM+)[4].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[183/200]
99	M	77	t-MDS/AML, MM,	D	17	45,XY,der(1)t(1; 17)(p36.1; q25),t(3; 17)(q26.2; q23),-7,del(20)(q13.1q13.3)[26]	ish t(3; 17)(5′MECOM+; 3′MECOM+)[3].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[172/200]
100	F	47	t-MDS	D	3	46,XX,add(3)(q27),t(3; 17)(q26.2; q23)[3]/46,XX[17]	ish add(3)(q27)(MECOM+),t(3; 17)(q26.2; q23)(5′MECOM+; 3′MECOM+)[1].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[150/200]
101	M	22	AML	D	10	47,XY,+X?c,t(3; 21)(q26.2; q22),del(13)(q12q14)[20]	ish t(3; 21)(q26.1; q22)(3′MECOM+,5′MECOM+)[2].nucish((MECOMx2)(3′MECOM sep 5′MECOMx1)[188/200]nucish(MECOMx2)[200]nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[178/200]
102	M	75	MDS + MCL	D	7	46,XY,r(7)(p11.2q11.2),der(12)del(12)(p11.2p12)inv(12)(p13q21)[3]/46,idem,t(3; 21)(q26.2; q22)[2]/46,XY[2]	ish t(3; 21)(q26.2; q22)(5′MECOM+; 3′MECOM+)[1].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[84/200]
103	F	59	MDS	D (SCT)	12	46,XX,t(3; 21)(q26.2; q22)[3]	ish t(3; 21)(3′MECOM+; 5′MECOM+).nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[104/200]
104	M	65	AML	D	9	46,XY,t(9; 17)(p22; q25),t(15; 15)(q21; q26)[7]/46,XY,idem,inv(3)(p25q12)[1]/48,XY,t(3; 21)(q26.2; q22),+13,+14[3]/46,XY[9]	ish t(3; 21)(q26.2; q22)(3′MECOM+; 5′MECOM+)[3].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[17/200]
105	M	82	AML/MDS	D	8	47,XY,t(3; 21)(q26.2; q22),+8[18]/48,idem,+mar[2]	ish t(3; 21)(q26.2; q22)(3′MECOM+; 5′MECOM+)[5].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[160/200]
106	M	67	MDS	D	5	46,XY,t(3; 21)(q26.2; q22),add(7)(q22)[13]/46,XY[7]	ish t(3; 21)(q26.2; q22)(3′MECOM+; 5′MECOM+)[3].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[59/200]
107	F	80	AML	D	15	46,XX,t(3; 21)(q26.2; q22)[1]/46,idem,t(2; 7)(q36; p15)[14]/46,XX[5]	nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECONx2)[191/200]
108	M	46	AML	D	2	46,XY,t(3; 21)(q26.2; q22),der(7)t(1; 7)(q21; q22)[6]/46,idem,+der(13; 21)(q10; q10),der(13; 21)(q10; q10)[14]	ish t(3; 21)(q26.2; q22)(MECOM+; 5′MECOM+)[4].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[181/200]
109	M	78	AML	D	10	46,XY,der(7; 18)(p10; q10),+11[2]/46,idem,t(3; 21)(q26.2; q22)[5]/45,X,-Y[5]/46,XY[8]	ish t(3; 21)(q26.2; q22)(MECOM+; 5′MECOM+)[4].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[50/200]
110	M	75	AML	A, NR	6	46,XY,t(3; 21)(q26.2; q22)[6]45,idem,-7[4]/46,XY[10]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[163/200]
111	F	63	MDS	A, PR	5	46,XX,t(3; 21)(q26.2; q22)[8]/46,idem,del(11)(p11.2)[5]/46,XX[4]	ish t(3; 21)(q26.2; q22)(MECOM+; 5′MECOM+)[2].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[173/200]
112	F	69	MDS, AML	D	0	46,XX,t(3; 21)(q26.2; q22),del(5)(q15q33),inv(9)(p12q13)[20]	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[175/200]
113	F	53	t-MDS/AML	A, PR	3	45,XX,t(3; 21)(q26.2; q22),-7[20]	nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[40/200]
114	M	71	AML/MDS	A, NR	2	46,XY,t(3; 21)(q26.2; q22)[15]/46,XY[5]	ish t(3; 21)(q26.2; q22)(MECOM+; 5′MECOM+)[3].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[173/200].nucish(RUNX1T1x2,RUNX1x3)[165/200]
115	M	39	t-MDS	A, PR	2	46,XY,t(3; 21)(q26.2; q22)[5]/46,idem,add(10)(p12)[1]/46,idem,inv(11)(p15q23)[1]/46,XY[13]	nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[176/200]
116	M	63	AML	D	7	46,XY,add(2)(p21),-3,-3,del(5)(q13q33),+8,add(17)(p11.2),add(22)(q13),+der(?)ins(?; 3)(?; q26.2q26.2)[3]/47,idem,+16[6]/45∼57,XY,add(2)(p21),-3,-3,del(5)(q13q33),+8,add(11)(p15),+16,add(17)(p11.2),add(22)(q13),+1∼5mar[cp11]	ish der(17)t(3; 17)(q22; p12)(MECOM+),der(?)ins(?; 3)(?; q26.2q26.2)(MECOM dim)[2]/der(17)t(3; 17)(q22; p12)(MECOM+),der(?)ins(?; 3)(?; q26.2q26.2)(3′MECOM+)[1].nuc ish(MECOMx1,MECOM dimx1)[463/500]/(3′MECOMx2,5′MECOMx1)(3′MECOM con 5′MECOMx1)[19/500]
117	F	69	AML	D	10	45,XX,der(1)ins(1; 3)(p22; q24q26.2)del(1)(q32),der(3)ins(1; 3)(p22; q24q26.2),del(5)(q22q33),-7,add(10)(p12)[19]/46,XX[1]	ish der(1)ins(1; 3)(p22; q24q26.2)del(1)(q32)(3′MECOM+),der(3)ins(1; 3)(3′MECOM-,5′MECOM+)[2].nuc ish(MECOMx2)(3′MECOM sep 5′MECOMx1)[194/200]
118	M	18	AML	D	2	38∼44,X,-Y,i(1)(q10),add(2)(p23),der(3)dup(3)(q23q27)add(3)(q29),-5,del(5)(q22q35),der(7)t(1; 7)(q21; p15),-9,add(10)(q22),add(15)(p11.2),der(16)t(?; 16)(?; p12)ins(?; 3)(?; q26.2q26.2),add(17)(p11.2),-18,-19,-21,+1∼3mar[cp20]	ish der(3)dup(3)(q23q27)(MECOM++)add(3)(q29), der(16)t(?; 16)(?; p12)ins(?; 3)(?; q26.2q26.2)(5′MECOM+)[2].nucish(3′MECOMx3,5′MECOMx4)(3′MECOM con 5′MECOMx3)[189/200]
119	M	53	AML	D	6	51,XY,del(3)(q26.2),+8,der(8)ins(8; 3)(q24.1; q26.2q26.2),t(9; 22)(q34; q11.2),add(11)(q23),+19,+20,+21,+der(22)t(9; 22)[20]	ish del(3)(q26.2)(3′MECOM+),der(8)ins(8; 3)(q24.1; q26.2q26.2)(5′MECOM+)[4].nuc ish(MECOMx2)(3′MECOM sep 5′MECOMx1)[195/200]
120	F	73	MDS	A, PR	7	46,XX,ins(12; 3)(p13; q21q26.2)[11]/46,XX[9]	ish ins(12; 3)(p13; q21q26.2)(MECOM+; 5′MECOM+)[2].nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[64/200].nucish(5′ETV6x2,3′ETV6x1)(5′ETV6 con 3′ETV6x1)[60/200]
121	F	39	AML	D	12	45,XX,ins(3; 3)(q21; q21q26.2),-7[14]//46,XY,inv(9)(p12q13)[6]	ish ins(3; 3)(q21; q21q26.2)(3′MECOM+,MECOM+; 5′MECOM+)[2].nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[79/200]
122	M	70	AML	D	1	41∼44,XY,der(3)del(3)(q25q26.2)add(3)(q27),der(3)del(3)(q25q26.2)hsr(3)(q26.2),-4,-5,-6,add(7)(p15),-12,-17,-21,add(22)(q11.2),+der(?)ins(?; 3)(?; q26.2q29)dup(3)(q26.2q29),+1∼3mar[cp20]	ish der(3)del(3)(q25q26.2)add(3)(q27)(3′MECOM-,5′MECOM+),der(?)ins(?; 3)(?; q26.2q29)(5′MECOM+)dup(3)(q26.2q29)(5′MECOM+)[1]/der(3)del(3)(q25q26.2)hsr(3)(q26.2)(3′MECOM-,5′MECOM amp)[2].nucish(MECOMx1,5′MECOM amp)[48/200]/(3′MECOMx1,5′MECOMx3∼6)(3′MECOM con 5′MECOMx1)[90/200]
123	M	50	AML	D	7	44,XY,add(4)(p16),del(5)(q13q33),add(8)(p21),add(8)(q24),add(17)(p12),-18,-19,-20,+mar[12]/44,XY,add(4)(p16),del(5)(q13q33),add(8)(q24),add(17)(p12),-18,-19,-20,+22[4]/42∼45,XY,add(4)(p16),del(5)(q13q33),add(8)(p21),add(8)(q24),add(17)(p12),-18,-19,-20,+1∼3mar[cp4].	nucish(3′MECOMx2,5′MECOMx3)(3′MECOM con 5′MECOMx2)[40/200]
124	M	78	AML	A, PR	0	45,XY,-7[19]/46,XY[1] (cryptic)	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[42/200]
125	F	69	AML	D	1	46,XX,add(3)(q21),inv(5)(p15q31)[20]	ish add(3)(q26)(3′MECOM+; 5′MECOM-)[2].nucish(3′MECOMx2,5′MECOMx1)(3′MECOM con 5′MECOMx1)[150/200]
126	M	74	CMML	D	9	46,XY,del(3)(q21q25)[5]/47,XY,+12[1]/46,XY[14]	ish del(3)(q21q25)(MECOM+)[2].nucish(3′MECOMx1,5′MECOMx2)(3′MECOM con 5′MECOMx1)[5/200]
127	M	54	AML	D	7	43-44,XY,-3,-4,add(5)(q11.2),-20,add(21)(q22),-22,+r,+1-2mar[cp20]	ish r(3′MECOM+,3′MECOM+,5′MECOM+,5′MECOM+)[3].nucish(MECOMx3)(3′MECOM sep 5′MECOMx2)[183/200]
128	F	18	t-MDS	D	0	n/a	nucish(MECOMx2)(3′MECOM sep 5′MECOMx1)[47/200]
129	M	60	MM; MDS	A, PR(SCT)	27	41,X,-Y,+1,der(1)t(1; 14)(p13; q12)t(14; 16)(q32; q23),del(1)(p34),del(1)(q22),t(3; ?)(q26.2; ?),del(6)(q14),der(7)t(7; 8)(p21; q12),add (7)(q36),del(8)(p22),-9,dic(9; ?)(q34; ?)t(?; 11)(?; q12),psu dic(10; 1)(q26; q12),-11,del(11)(q12),-13,-14,-15,-15,der(16)t(14; 16),add(16)(q12),-17,+19,add(19)(p13),add(21)(p13)x2,-22,+1∼4mar[cp14]/46,XY[6]	nucish(3′ MECOMx2,5′ MECOMx3)(3′ MECOM con 5′ MECOMx2)(MECOM amp)[100/200]

MM: multiple myelom; cryptic: can't be detected by chromosomal analysis

General information, karyotyping and MECOM FISH results in 129 cases of myeloid malignancies. MM: multiple myelom; cryptic: can't be detected by chromosomal analysis

Experimental design, materials and methods

This is a retrospective study. A search of the cytogenetics database for MECOM FISH positive cases from May 1, 2009 to August 15, 2018 at The University of Texas MD Anderson Cancer Center (MDACC) has been performed, and a total of 129 cases with at least one time MECOM FISH positive result has been found. Other cases with 3q26 abnormality by conventional cytogenetics but negative for and/or not been confirmed for MECOM rearrangement are excluded from this study. Clinicopathologic data, including MECOM FISH test results, were collected by electronic medical chart review. Conventional G-banded chromosomal analysis (karyotyping) has been routinely performed on unstimulated 24-h and 48-h BM aspirate cultures using standard techniques as we have reported previously [6], [7], [8]. Interphase-, metaphase-, map-back FISH and WCP have been performed by following existing laboratory protocols as reported previously [6], [7], [8], [9]. The following probe/probe set have been employed for this study. The MECOM (EVI1) dual color, breakapart (BAP) DNA probe (#KI-10204) from Leica Biosystems/Kreatech (Buffalo Grove, IL; theVysis LSI BCR/ABL ES Dual Color Fusion probe, VysisMYC BAP probe; the Vysis ETV6 BAP Probe and the VysisRUNX1T1/RUNX1 Fusion probe (Abbott Molecular, Des Plaines, IL).All these probe/probe set used for our clinical diagnosis have been validated in our laboratory in accord with the American College of Medical Genetics and Genomics (ACMGG) guidelines.

Specifications Table

Subject area	Biology
More specific subject area	tumor
Type of data	Table
How data was acquired	Microscope, The CytoVision 7.6 (Leica Biosystems)
Data format	Raw data
Experimental factors	Chromosomal analysis and FISH analysis on cultured bone marrow specimen by following standard clinical laboratory protocol
Experimental features	Conventional Cytogenetics and FISH analyses
Data source location	Houston, Texas, United State of America
Data accessibility	Data is with this article
Related research article	Z. Tang, G. Tang, S. Hu, K.P. Patel, C.C. Yin, W. Wang, P. lin, G.A. Toruner, C.Y. Ok, J Gu, X. Lu, J.D. Khoury and L.J. Medeiros. Deciphering the Complexities of MECOM Rearrangement-Driven Chromosomal Aberrations. Cancer Genet. 233–234 (2019) 21–31. https://doi.org/10.1016/j.cancergen.2019.03.002

Value of the data

•

This dataset provides so far the largest number of cases from a single institute about MECOM rearrangement in myeloid malignancies [1]. Scientists and/or pathologists in the field of cytogenetic and genomic diagnostics can use these cases as references for complex cases involving chromosome 3 abnormalities.

•

The MECOM rearrangement has been confirmed by FISH analysis in all cases, comparing to cases with chromosome 3 abnormalities but without further confirmation of a MECOM rearrangement in previous studies [2], [3], [4], [5], [6].

•

The majority of cases with atypical chromosomal abnormalities have been thoroughly analyzed by interphase-, metaphase-, map-back FISH and/or WCP. Therefore, the results, especially the ISCN description of all chromosomal aberrations, are accurate and reliable.

9 in total

1. t(3;8)(q26.2;q24) Often Leads to MECOM/MYC Rearrangement and Is Commonly Associated with Therapy-Related Myeloid Neoplasms and/or Disease Progression.

Authors: Guilin Tang; Shimin Hu; Sa A Wang; Wei Xie; Pei Lin; Jie Xu; Gokce Toruner; Ming Zhao; Jun Gu; Madison Doty; Shaoying Li; L Jeffrey Medeiros; Zhenya Tang
Journal: J Mol Diagn Date: 2018-12-19 Impact factor: 5.568

Review 2. 3q26/EVI1 rearrangements in myeloid hemopathies: a cytogenetic review.

Authors: Marc De Braekeleer; Marie-Josée Le Bris; Etienne De Braekeleer; Audrey Basinko; Frédéric Morel; Nathalie Douet-Guilbert
Journal: Future Oncol Date: 2015 Impact factor: 3.404

3. Deciphering the complexities of MECOM rearrangement-driven chromosomal aberrations.

Authors: Zhenya Tang; Guilin Tang; Shimin Hu; Keyur P Patel; C Cameron Yin; Wei Wang; Pei Lin; Gokce A Toruner; Chi Y Ok; Jun Gu; Xinyan Lu; Joseph D Khoury; L Jeffrey Medeiros
Journal: Cancer Genet Date: 2019-03-11

4. Conventional cytogenetics and breakpoint distribution by fluorescent in situ hybridization in patients with malignant hemopathies associated with inv(3)(q21;q26) and t(3;3)(q21;q26).

Authors: Etienne De Braekeleer; Nathalie Douet-Guilbert; Audrey Basinko; Clément Bovo; Nadia Guéganic; Marie-Josée Le Bris; Frédéric Morel; Marc De Braekeleer
Journal: Anticancer Res Date: 2011-10 Impact factor: 2.480

5. High EVI1 expression predicts outcome in younger adult patients with acute myeloid leukemia and is associated with distinct cytogenetic abnormalities.

Authors: Stefan Gröschel; Sanne Lugthart; Richard F Schlenk; Peter J M Valk; Karina Eiwen; Chantal Goudswaard; Wim J L van Putten; Sabine Kayser; Leo F Verdonck; Michael Lübbert; Gert-Jan Ossenkoppele; Ulrich Germing; Ingo Schmidt-Wolf; Brigitte Schlegelberger; Jürgen Krauter; Arnold Ganser; Hartmut Döhner; Bob Löwenberg; Konstanze Döhner; Ruud Delwel
Journal: J Clin Oncol Date: 2010-03-22 Impact factor: 44.544

6. Clinical, molecular, and prognostic significance of WHO type inv(3)(q21q26.2)/t(3;3)(q21;q26.2) and various other 3q abnormalities in acute myeloid leukemia.

Authors: Sanne Lugthart; Stefan Gröschel; H Berna Beverloo; Sabine Kayser; Peter J M Valk; Shama Lydia van Zelderen-Bhola; Gert Jan Ossenkoppele; Edo Vellenga; Eva van den Berg-de Ruiter; Urs Schanz; Gregor Verhoef; Peter Vandenberghe; Augustin Ferrant; Claus-Henning Köhne; Michael Pfreundschuh; Heinz A Horst; Elisabeth Koller; Marie von Lilienfeld-Toal; Martin Bentz; Arnold Ganser; Brigitte Schlegelberger; Martine Jotterand; Jürgen Krauter; Thomas Pabst; Matthias Theobald; Richard F Schlenk; Ruud Delwel; Konstanze Döhner; Bob Löwenberg; Hartmut Döhner
Journal: J Clin Oncol Date: 2010-07-26 Impact factor: 44.544

7. Breakpoint heterogeneity in (2;3)(p15-23;q26) translocations involving EVI1 in myeloid hemopathies.

Authors: Marc De Braekeleer; Nadia Guéganic; Corine Tous; Marie-Josée Le Bris; Audrey Basinko; Frédéric Morel; Nathalie Douet-Guilbert
Journal: Blood Cells Mol Dis Date: 2014-11-26 Impact factor: 3.039

8. Genomic aberrations involving 12p/ETV6 are highly prevalent in blastic plasmacytoid dendritic cell neoplasms and might represent early clonal events.

Authors: Zhenya Tang; Yan Li; Wei Wang; C Cameron Yin; Guilin Tang; Phyu P Aung; Shimin Hu; Xinyan Lu; Gokce A Toruner; L Jeffrey Medeiros; Joseph D Khoury
Journal: Leuk Res Date: 2018-09-17 Impact factor: 3.156

9. Double inv(3)(q21q26.2) in acute myeloid leukemia is resulted from an acquired copy neutral loss of heterozygosity of chromosome 3q and associated with disease progression.

Authors: Jun Gu; Keyur P Patel; Bing Bai; Ching-Hua Liu; Guilin Tang; Hagop M Kantarjian; Zhenya Tang; Ronald Abraham; Rajyalakshmi Luthra; L Jeffrey Medeiros; Pei Lin; Xinyan Lu
Journal: Mol Cytogenet Date: 2015-08-19 Impact factor: 2.009

9 in total