Carbon Dioxide Improves Phosphorus Nutrition by Facilitating the Remobilization of Phosphorus From the Shoot Cell Wall in Rice (Oryza sativa).

Phosphorus (P) starvation leads to increased reutilization of cell wall P in rice (Oryza sativa). Carbon dioxide (CO2) is involved not only in plant growth and development but also in the response to abiotic stresses. However, it remains unclear whether CO2 affects the reutilization of cell wall P in rice when subjected to P deficiency. In the present study, elevated CO2 (600 μl·L-1) significantly increased the soluble P content in shoots when compared with ambient CO2 (400 μl·L-1). This positive effect was accompanied by an increase of pectin content, as well as an increase of pectin methylesterase (PME) activity, which results in P release from the shoot cell wall, making it available for plant growth. P deficiency significantly induced the expression of phosphate transporter genes (OsPT2, OsPT6, and OsPT8) and decreased the P content in the xylem sap, but elevated CO2 had no further effect, indicating that the increased soluble P content observed in shoots under elevated CO2 is attributable to the reutilization of shoot cell wall P. Elevated CO2 further increased the P deficiency-induced ethylene production in the shoots, and the addition of the ethylene precursor 1-amino-cyclopropane-1-carboxylic acid (ACC) mimicked this effect, while the addition of the ethylene inhibitor aminoethoxyvinylglycine (AVG) abolished this effect. These results further support the role of ethylene in the alleviation of P deficiency under elevated CO2. Taken together, our results indicate that the improvement of P nutrition in rice by elevated CO2 is mediated by increasing the shoot cell wall pectin content and PME activity, possibly via the ethylene signaling pathway.

Introduction

Inorganic phosphate (Pi), the main form of pan> class="Chemical">phosphorus (P) taken up by plants, is essential for the normal growth of plants (Wu et al., 2013). However, due to the chemical fixation of P to cations such as Al and Fe, or to other compounds, which creates insoluble, inaccessible complexes, P deficiency is one of the major limiting factors for crop production (Vance et al., 2003; Bhadouria et al., 2017). As a consequence, large amounts of Pi fertilizers are needed (Hammond et al., 2004). As Pi-based fertilizers originate from rock phosphate and other non-renewable materials, overuse of Pi will not only exhaust the mineral P resources (Runge-Metzger, 1995; Beardsley, 2011) but also cause damage to the environment (Liang et al., 2014), such as lake eutrophication (Withers et al., 2001). Thus, breeding crops with higher yields and lower fertilization requirements is imperative for sustainable agriculture.

Plants have developed flexible strategies to cope with P limitation, such as modification of root architecture, symbiosis with mycorrhizal funpan>gi, secretion of pan> class="Chemical">organic acids, modification of carbon metabolism, remobilization of Pi, and enhancing the biosynthesis of phosphate transporters (Raghothama, 1999; Smith, 2001; Shulaev et al., 2008). The cell wall also plays a key role in response to P deficiency. For example, in rice (Oryza sativa), the P stored in the root cell wall can be reutilized under P starvation conditions (Zhu et al., 2015). Cell walls consist of cellulose and other matrix polysaccharides such as pectin and hemicellulose (Cosgrove, 2005). Among them, only pectin has been demonstrated to participate in the reutilization of cell wall P (Zhu et al., 2015), as pectin harbors negatively charged carboxylic groups (–COO–) that have high affinity for Cd2+, Fe3+, and Al3+ (Blamey et al., 1990; Chang et al., 1999; Rengel and Zhang, 2003). Phosphate (PO43−) also has high affinity for Ca2+, Fe3+, and Al3+, and as a result, insoluble FePO4, AlPO4, and Ca3(PO4)2 are formed. For instance, under aluminum stress, AlPO4 is the major form of P in the apoplast in buckwheat (Fagopyrum esculentum; Zheng et al., 2005). As the negatively charged carboxylic groups in pectin can chelate Fe3+, PO43− can be further trapped with a linkage of –COO–Fe–PO4. That means, ions such as Fe act as bridges connecting pectin and P; therefore, increasing the –COO– will bind Fe more tightly to the pectin and facilitate the release of pectin-trapped PO43− (Zhu et al., 2015). Furthermore, this pectin-mediated P reutilization is regulated by a series of phytohormones and signaling molecules, such as nitric oxide (NO; Zhu et al., 2017), abscisic acid (ABA; Zhu et al., 2018), and ethylene (Zhu et al., 2016). However, it remains unclear whether other signal molecules are involved in this cell wall P remobilization mechanism.

Carbon dioxide (pan> class="Chemical">CO2) is one of the gases responsible for global climate change (IPCC 2001). Atmospheric CO2 has increased more than 40% from the pre-industrial times, and current ambient CO2 now exceeds 400 μmol mol−1 (Tans and Keeling, 2018) and is expected to reach a peak of 490–1,260 μl·L−1 in the future (Houghton et al., 2001). The increase of CO2 in the atmosphere alters the growth and development of plants, which could also influence the absorption of macro- or micronutrients. Previous studies have focused on the influence of elevated CO2 on the plant population, community, ecosystem, and physiological scales (Gibeaut et al., 2001; Teng et al., 2006; Niu et al., 2011), while few studies have focused on the influence of elevated CO2 on the utilization of nutrients. CO2 may be involved in nutrient acquisition in plants. For example, the demand for P in Japanese red pine (Pinus densiflora) is related to the concentration of CO2 (Kogawara et al., 2006), while elevated concentrations of CO2 can alleviate the iron deficiency phenotype of tomato (Solanum lycopersicum; Jin et al., 2009). In addition, Niu et al. (2013) reported that the combination of elevated CO2 and nitrate can alleviate P-deficiency symptoms in Arabidopsis thaliana (L.) Heynh through alteration of morphological and physiological responses. Furthermore, Zhu et al. (2017) demonstrated that elevated CO2 can alleviate Al toxicity in rice by decreasing the binding capacity of the cell wall to Al. As about 49% of the total P was present in the cell wall of rice (Zhu et al., 2016), that means, the cell wall has abundant P resource for reutilization; thus the relationship among elevated CO2, P status, and cell walls is worth discussing and studying.

Rice is anpan> importanpan>t staple food worldwide, which pan> class="Chemical">accounts for approximately 21% of the caloric supply for the world’s population (Wu et al., 2011). Low P in soils significantly limits rice production (Kirk et al., 1998). Therefore, improving the utilization efficiency of P in rice is an important target for sustainable agriculture. The objective of the current study was to clarify whether elevated CO2 is involved in the reutilization of cell wall P in P-deficient conditions.

Materials and Methods

Plant Materials and Growth Conditions

Rice (pan> class="Species">Oryza sativa) subsp. japonica “Nipponbare” (Nip) was used in the current study. After soaking in 1% (v/v) sodium hypochlorite (NaClO) for 10 min, seeds were immediately rinsed with deionized water and then soaked in ultrapure water until germination. The germinated seeds were cultivated in calcium chloride solution (CaCl2; 0.5 mM) until the buds were about 1 cm long. Then, the seedlings were transferred to modified Kimura B nutrient solution for 2 weeks (Zhu et al., 2018). Seedlings of similar size were then transplanted into a 1.25-L plastic pot (4 seedlings per pot) and subjected to the ambient CO2 (400 μl·L−1) or elevated CO2 (600 μl·L−1) condition in the presence or absence of P, termed as +P, +P + CO2, −P, and −P + CO2. To investigate the crosstalk between elevated CO2 and ethylene, the following treatments were used: +P, +P + ACC (1-amino-cyclopropane-1-carboxylic acid), +P + AVG (aminoethoxyvinylglycine), +P + CO2, +P + CO2 + ACC, +P + CO2 + AVG, −P, −P + ACC, −P + AVG, −P + CO2, −P + CO2 + ACC, and −P + CO2 + AVG. The final concentration of ACC was 10 μM and that of AVG was 0.2 μM. The pH of the nutrient solution was 5.5.

Measurement of the Soluble P Content

After measuring the fresh weights of roots and shoots, samples were rinsed with ultrapure water anpan>d thenpan> grounpan>d in liquid pan> class="Chemical">nitrogen. After extracting with 8 ml of 5 M H2SO4 for 2 h, samples were centrifuged at 12,000g for 8 min, and 400 μl of supernatant was transferred to a 2 ml Eppendorf tube and mixed with 200 μl of ammonium molybdate (containing 15% ascorbic acid, pH 5.0) at 37°C for 0.5 h. Finally, the absorbance of the above mixture was detected at 650 nm and the P content was normalized by fresh weight (Zhu et al., 2018).

Measurement of Total P Content

n class="Species">Rice seedlings were first rinsed with distilled n class="Chemical">water, then separated into roots and shoots, and weighed. After the samples were dried in an oven at 75°C for 3 days, 4 ml of HNO3 was added. The above mixture was incubated at 130°C for 24 h, and after cooling, ultrapure water was added to reach a final volume of 20 ml. The P concentration in the solution was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES; Fisons ARL Accuris, Ecublens, Switzerland).

Measurement of the P Concentration in Xylem Sap

n class="Species">Rice seedlings were first excised with a razor 2 cm above the root, and then the xylem sap was collected (Che et n class="Chemical">al., 2016). Two hours later, the volume of the xylem sap was recorded, and 1 ml of distilled water was added for the measurement of P content by ICP-AES.

Extraction of Cell Wall and Cell Wall Pectin

For cell wall extraction, roots were first grounpan>d in liquid pan> class="Chemical">nitrogen and 8 ml of 75% ethanol was added. After incubation at 4°C for 20 min, samples were centrifuged at 12,000g for 20 min and the pellet was collected. Next, 8 ml of acetone was added to the pellet, and after a 20 min incubation, the supernatant was removed as described above and the pellet was extracted with 8 ml of chloroform:methyl alcohol (1:1) and again with 8 ml of methyl alcohol. Finally, the pellet, containing cell wall material, was dried in a freeze dryer.

n class="Chemical">Pectin was extracted as follows: 1 ml of 100°C ultrapure n class="Chemical">water was added to about 5 mg of the extracted cell wall and incubated for 1 h. Next, the mixture was centrifuged at 12,000g for 8 min and the supernatant was collected. The pellet was extracted with 1 ml ultrapure water two more times and the supernatants were collected as described above. The collected supernatants were regarded as pectin solution (Zhong and Lauchli, 1993).

Measurement of the Uronic Acid Content in Pectin

A 200 μl n class="Chemical">aliquot of the extracted n class="Chemical">pectin solution was transferred to a 2 ml tube, then 1 ml of 98% H2SO4 (consisting of 12.5 mM Na2B4O7·10H2O) was added. After incubation in a 100°C water bath for 5 min, samples were chilled, and 20 μl of 0.15% M-hydro-diphenyl (m:v) was added. Finally, the mixture was incubated at 25°C for 20 min and the absorbance was measured at 520 nm (Blumenkrantz and Asboe-Hansen, 1973). Galacturonic acid (Sigma) was used as a standard.

Measurement of the Pectin Methylesterase Activity

About 5 mg of cell wall materials were weighed, and then 1 ml of 1 M NaCl (dissolved in 10 mM Tris buffer, pH 6.0) was added to extract the PME enzyme at 4°C. Then, the mixture was centrifuged at 12,000g for 15 min and the supernatant was collected. Next, 0.1 ml of 200 mM PBS solution (containing 640 μg ml−1 pectin; pH 7.5) and 10 μl of alcohol oxidase were added and incubated at 30°C for 10 min. Then, 0.2 ml of 0.5 M NaOH (containing 5 mg ml−1 purpald) was added and the mixture was incubated at 30°C for 30 min. Finally, the absorbance was measured at 550 nm (Zhu et al., 2016).

Measurement of the Cell Wall P Content

To measure the cell wn class="Chemical">all P content, 1 ml of 2 M n class="Chemical">HCl was added to 5 mg of cell wall extract and allowed to sit for 24 h with occasional shaking. The mixture was then centrifuged at 13,000g for 5 min and the supernatant was collected, and ultrapure water was added to bring to a final volume of 5 ml. The P content was measured by ICP-AES.

Measurement of Ethylene Production

For determination of ethylene production, shoots were collected after the treatmenpan>ts anpan>d placed into 8 ml glass vials and rapidly sealed as reported by Wu et al. (2011). Then, shoot samples were incubated at 25°C in the dark for 4 h. Gas (1 ml) was withdrawn from the airspace of each vial using a gas-tight syringe (Focus GC, Thermo, USA) and injected into a gas chromatograph (Focus GC, Thermo, USA) equipped with a capillary column (CP-carboPLOT P7, Varian, CA, USA) and flame-ionization detector.

Measurement of the Photosynthesis Rate

The net photosynthesis rate of the youngest fully expanded leaf was measured with a LI-6400 Infrared Gas Analyzer (Li-Cor Bioscienpan>ces) using a red-blue light at a PAR intenpan>sity of 1,200 μmol photons m−2 s−1, a constanpan>t pan> class="Chemical">CO2 concentration of 400 μl L−1, a 25°C leaf temperature, and 40% relative humidity (Sun et al., 2018).

Gene Expression Analysis

After the treatments, roots were collected and instantly frozen in liquid nitrogen. The extraction of totpan> class="Chemical">al RNA, the conversion of the total RNA to cDNA, and the subsequent quantitative PCR were all conducted according to Zhu et al. (2016). The PCR reaction mixture consisted of RNase-free water (2.6 μl), cDNA (2 μl), SYBR Premix (5 μl, Toyobo, Japan), forward primer (0.2 μl), and revise primer (0.2 μl). The primers are listed in Table 1 (Zhu et al., 2016). Three replications were used for each cDNA sample, and the relative expression of the candidate genes was calculated by the 2-ΔΔCT method (Livak and Schmittgen, 2001). OsHistone was used as a reference gene (Xia et al., 2013).

Table 1

Gene-specific primers used in this work.

Gene	Forward (5′-3′)	Reverse (5′-3′)
OsPT2	GACGAGACCGCCCAAGAAG	TTTTCAGTCACTCACGTCGAGAC
OsPT6	TATAACTGATCGATCGAGACCAGAG	TGGATAGCCAGGCCAGTTATATATC
OsPT8	AGAAGGCAAAAGAAATGTGTGTTAAAT	AAAATGTATTCGTGCCAAATTGCT
OsHistone H3	GGTCAACTTGTTGATTCCCCTCT	AACCGCAAAATCCAAAGAACG

Statistical Analysis

Three replications were used for each set of experiments. Data were analyzed by one-way anpan>pan> class="Chemical">alysis of variance (ANOVA) and the means were compared by Duncan’s multiple range test. Different letters on the histograms indicate that the means were statistically different at p < 0.05.

Results

Elevated CO2 Improves the Growth of Rice Seedlings

To investigate the response of rice to pan> class="Disease">P deficiency under elevated CO2, 2-week-old rice seedlings were grown in either a P-sufficient or P-deficient solution in ambient or elevated CO2 for 7 days. As shown in Figures 1A,B, P deficiency reduced the growth of roots and shoots; however, shoot growth was improved under elevated CO2, independent of the P status, when compared with ambient CO2, while root growth was nearly the same in both ambient and elevated CO2 treatments. These results indicated that elevated CO2 can improve shoot growth. Then, a question raised why elevated CO2 can improve the growth of shoots in both P-sufficient and P-deficient conditions. As CO2 is an important substrate molecule for photosynthesis, and higher concentrations of CO2 would affect the photosynthesis rate, we wondered whether elevated CO2 could alleviate the reduction of rice plant growth caused by P deficiency. As shown in Supplementary Figure 1, while P deficiency significantly decreased the photosynthesis rate, elevated CO2 significantly promoted the photosynthesis rate, indicating that the enhanced shoot growth observed under elevated CO2 may be related to an enhanced photosynthesis rate. Moreover, soluble P accumulated in the shoot under elevated CO2 (Figure 1D); however, no alteration was found in the root soluble P content (Figure 1C), indicating that elevated CO2 can stimulate more efficient reutilization of P in shoots. However, under P deficiency, no significant difference in the total P content was found between elevated CO2 and ambient CO2 conditions (Supplementary Figure 2); the underlying reason may be that the plants failed to take up external P under the −P conditions.

Figure 1

Effects of elevated CO2 on the biomass of rice roots and shoots (A,B) and the soluble P content in rice roots and shoots (C,D) grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Efn class="Chemical">fects of elevated n class="Chemical">CO2 on the biomass of rice roots and shoots (A,B) and the soluble P content in rice roots and shoots (C,D) grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Elevated CO2 Significantly Increased the Reutilization of Shoot Cell Wall P

We questioned whether the P that accumulated in the shoot unpan>der elevated pan> class="Chemical">CO2 originated from elevated P translocation from the roots or from elevated P reutilization from the shoot cell wall, as the cell wall acts as a P pool. Thus, we analyzed the expression of three phosphate transporter genes (OsPT2, OsPT6, and OsPT8) that are responsible for root-to-shoot P translocation. As shown in Figure 2, P deficiency significantly induced the expression of OsPT2, OsPT6, and OsPT8 under the ambient CO2 condition (−P), while elevated CO2 (-P + CO2) had no further effect, which was also observed in the P-sufficient conditions, indicating that root-to-shoot P translocation may not be involved in the elevated soluble P content observed in shoots under elevated CO2. To clarify whether elevated CO2 stimulates root-to-shoot P translocation, we also measured the P content in xylem sap. Although significantly less P was observed in xylem sap when rice was grown under the P-deficient conditions, elevated CO2 had no further effect (Supplementary Figure 3). In addition, we found no alteration of the root cell wall P content (Figure 3A), while the P content in the shoot cell wall was decreased under elevated CO2 (Figure 3B). These results further suggested that the elevated soluble P content observed in shoots under elevated CO2 is attributed to the reutilization of shoot cell wall P.

Figure 2

Effect of elevated CO2 on the expression of OsPT2 (A), OsPT6 (B), and OsPT8 (C) in rice roots grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Figure 3

Effects of elevated CO2 on root cell wall P content (A) and shoot cell wall P content (B) in rice grown under +P and −P conditions for 1 week. Data are means ± SD (n = 44). Columns with different letters are significantly different at p < 0.05.

Efn class="Chemical">fect of elevated n class="Chemical">CO2 on the expression of OsPT2 (A), OsPT6 (B), and OsPT8 (C) in rice roots grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Efn class="Chemical">fects of elevated n class="Chemical">CO2 on root cell wall P content (A) and shoot cell wall P content (B) in rice grown under +P and −P conditions for 1 week. Data are means ± SD (n = 44). Columns with different letters are significantly different at p < 0.05.

Elevated CO2 Significantly Increased the Shoot Cell Wall Pectin Content

Previous studies demonstrated that pectin contributes greatly to cell wpan> class="Chemical">all P reutilization in rice (Zhu et al., 2015); thus, we examined the role of pectin in rice under P-deficiency and elevated CO2 conditions. As expected, less P was adsorbed in shoot cell wall pectin under elevated CO2 compared to ambient CO2, indicating a greater P-release potential in the pectin fraction under elevated CO2 (Figure 4A). In agreement with this, we found an increase of the pectin content under elevated CO2, irrespective of the P status (Figure 4B). However, considering that about 88% of pectin-released P accounts for the net soluble P gained in the shoot under the −P condition (As shown in Figure 4A, the shoot cell wall pectin-retained P was decreased from 1.13 μg mg−1 cell wall in the −P treatment to 0.85 μg mg−1 cell wall in the −P + CO2 treatment, while as shown in Figure 1D, the shoot soluble P was increased from 125.9 μg g−1 fresh weight in the −P treatment to 151.4 μg g−1 fresh weight in the −P + CO2 treatment. As 1 g of fresh shoot can provide about 80 mg cell wall, thus about 22.4 μg P was released from 80 mg Nip cell wall pectin in the −P + CO2 treatment when compared with −P treatment alone, which accounts for 88% of the net soluble P gained – 25.5 μg g−1 fresh weight in the −P + CO2 treatment when compared with −P treatment. The almost complete absence of any change in the cell wall pectin content and its P retention in rice roots under both ambient and elevated CO2 conditions, this again excludes the involvement of the roots in the increase of P reutilization under elevated CO2 (Supplementary Figure 4), Thus, we focused on shoots instead of roots in the following examinations.

Figure 4

Effects of elevated CO2 on P content (A) and uronic acid content in shoot cell wall pectin (B) in rice grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Efn class="Chemical">fects of elevated n class="Chemical">CO2 on P content (A) and uronic acid content in shoot cell wall pectin (B) in rice grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Elevated CO2 Significantly Increased the Pectin Methylesterase Activity in the Shoot

Only de-esterified pectin will presenpan>t a net negative charge in the cell wpan> class="Chemical">all space, and this negative charge is generated from the demethylation of pectin, a process that controlled by PME. Thus, we measured the PME activity in the shoot cell wall. As shown in Figure 5, P deficiency significantly induced the PME activity in the shoot cell wall, and this increment was further amplified under elevated CO2, indicating that the negative charges generated by PME are also involved in the increased reutilization of shoot cell wall P under elevated CO2.

Figure 5

Effects of elevated CO2 on PME activity in shoot cell wall in rice grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Efn class="Chemical">fects of elevated n class="Chemical">CO2 on PME activity in shoot cell wall in rice grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Elevated CO2 Significantly Increased Ethylene Production in Rice Shoots

We next questioned how elevated CO2 canpan> increase the reutilization of shoot cell wpan> class="Chemical">all P. As ethylene is a signal that is closely related to the P reutilization in the rice cell wall (Zhu et al., 2016, 2017), we hypothesized that there could be a relationship between elevated CO2 and ethylene. As expected, P deficiency significantly induced ethylene production in rice roots, and elevated CO2 further induced this increment of ethylene production (Figure 6). To determine whether this induction of ethylene production is involved in the accelerated P reutilization observed under elevated CO2, we applied a precursor of ethylene (ACC) and an inhibitor of ethylene (AVG). As shown in Figure 7, the ACC treatment had a similar effect as that of the elevated CO2 treatments in that we found more shoot soluble P under the ACC treatment irrespective of the P status, while the results were opposite for the AVG treatment, which further confirmed the role of ethylene in the increased reutilization of shoot cell wall P under elevated CO2.

Figure 6

Effects of elevated CO2 on ethylene production in rice shoots grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Figure 7

Effects of different treatments on the soluble P content in rice roots (A,C) and shoots (B,D) grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Efn class="Chemical">fects of elevated n class="Chemical">CO2 on ethylene production in rice shoots grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Efn class="Chemical">fects of difn class="Chemical">ferent treatments on the soluble P content in rice roots (A,C) and shoots (B,D) grown under +P and −P conditions for 1 week. Data are means ± SD (n = 4). Columns with different letters are significantly different at p < 0.05.

Discussion

Elevated CO2 canpan> afpan> class="Chemical">fect plant growth by increasing the requirements for macronutrients, such as P and nitrogen (N; Kogawara et al., 2006; Lagomarsino et al., 2008), and also by improving P nutrition (Niu et al., 2013); facilitating the reutilization of iron (Fe; Jin et al., 2009); and alleviating Al toxicity in rice (Zhu et al., 2017). However, how elevated CO2 facilitates the reutilization of P in rice remains elusive. In the present study, we found that elevated CO2 can stimulate more efficient reutilization of shoot cell wall P (Figure 1), as well as increase the pectin content and PME activity of the shoot cell wall (Figures 4, 5), which in turn releases cell wall P (Figure 3), resulting in more soluble P in the rice shoot (Figure 1). Furthermore, ethylene appears to participate in this process (Figures 6, 7). To our knowledge, this is the first study to show that elevated CO2 can affect the reutilization of shoot cell wall P in rice.

As the P in cell walls pan> class="Chemical">accounts for approximately 50% of the root total P in rice (Zhu et al., 2015), any approaches to improve the reutilization of the P from the cell wall may promote plant growth under P-deficient conditions in rice. Studies in rice have shown a high correlation of the cell wall-released P and the root/shoot soluble P content (Zhu et al., 2015, 2016, 2018). Moreover, Yu et al. (2016) recently identified one Arabidopsis mutant, which lacks the function of AMMONIUM-OVERLY-SENSITIVE1, amos1, that released more P from its root cell wall under P-deficient conditions and thus was highly resistant to P deficiency. In this study, elevated CO2 significantly increased the release of P from the rice shoot cell wall (Figure 3), which in turn increased the soluble P content in rice shoots (Figure 1), indicating a cell wall P-based P reutilization mechanism. Furthermore, as the pectin content is the major factor that positively determines the efficiency of the release of cell wall P under P deficiency (Zhu et al., 2015), we investigated whether pectin contributed to the increased reutilization of cell wall P. As expected, elevated CO2 significantly increased the pectin content and decreased P retention within this polysaccharide under the −P condition (Figure 4), indicating that pectin contributes to the increased P reutilization in rice shoot cell walls under elevated CO2.

Pectin is a major pan> class="Chemical">polysaccharide in the cell wall and consists of rhamnogalacturonan I (RG I), rhamnogalacturonan II (RG II), galactans, arabinans, homogalacturonan (HGA), and other polysaccharides (Cosgrove, 2005). Several studies have indicated that the negatively charged carboxylic groups in the D-galacturonic acid component of the pectic network of plant cell walls have a high affinity for Al3+ and Fe3+; thus, this network possesses the ability to solubilize Al-bound P and Fe-bound P in the cell wall, and as a result, the insoluble P can be reutilized (Ae et al., 1996; Gessa et al., 1997). Zhu et al. (2015) demonstrated that pectin participates in the cell wall P reutilization mechanism by comparing the pectin content and root soluble P content in 14 different rice cultivars cultured in −P nutrient solution for 1 week. In addition, homogalacturonans, the main component of pectin, are first synthesized in the Golgi apparatus in the methyl esterified form. After secretion into the cell wall, the demethylation process of pectin occurs (the removal of -CH3- from carboxyl groups), which is catalyzed by PME (Ostatek and Blamey, 1995; Ridley et al., 2001). This results in high amounts of negatively charged groups, and as a result, the binding capacity to cations is enhanced (Cosgrove, 2005; Willats et al., 2006). Therefore, the higher the PME activity, the more negatively charged groups in pectin, and more P is released from the cell wall. As expected, in the present study, elevated CO2 significantly increased the PME activity in the P-deficient rice shoots (Figure 5), indicating that elevated CO2 enhances the remobilization of cell wall P in rice shoots by stimulating pectin biosynthesis and increasing PME activity.

Roots act as a P pool for the shoots. Once P is released from the cell wall to the symplasm, three pan> class="Chemical">phosphate transporters, OsPT2, OsPT6, and OsPT8, are responsible for the translocation of P to the shoots via symplasmic transport to the xylem parenchyma cells, followed by secretion to the xylem (Jeschke et al., 1997; Ai et al., 2009; Jia et al., 2011). It is interesting that although P deficiency significantly induced the expression of the genes encoding these phosphate transporters, elevated CO2 had no further effect (Figure 2). The none alteration of the xylem P content between −P and −P + CO2 treatment further indicated that the increased soluble P found in the shoot was not derived from the root (Supplementary Figure 3).

Then, how does elevated CO2 increase the shoot cell wpan> class="Chemical">all pectin content and then increase P reutilization? Ethylene has been demonstrated to be a key molecule involved in P deficiency, such as facilitating P reutilization in rice (Zhu et al., 2016, 2017). Here, elevated CO2 significantly increased the P deficiency-induced production of ethylene in the shoots (Figure 6), and the addition of the ethylene precursor ACC and the inhibitor of ethylene (AVG) made no difference of the shoot soluble P content between the ambient and elevated CO2 conditions (Figure 7), indicating that the effect of elevated CO2 on rice under P deficiency may be related to the increased production of ethylene.

In conclusion, we have demonstrated that elevated CO2 canpan> increase the reutilization of shoot P in pan> class="Species">rice. This reutilization is mediated by a cell wall P-based P remobilization mechanism involving an increase of pectin content and PME activity. Ethylene may act downstream of CO2 to control these processes; however, this putative mechanism needs further clarification.

Author Contributions

XFZ, XLZ, and XYD performed the research. XFZ ann class="Chemical">alyzed the data and wrote the draft. XFZ and RFS designed the research. RFS wrote the article.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercin class="Chemical">al or financin class="Chemical">al relationships that could be construed as a potential conflict of interest.