| Literature DB >> 31189664 |
Robert A Bradley1,2, Jack Shireman1, Caya McFalls1, Jeea Choi3, Scott G Canfield4,5, Yi Dong1, Katie Liu1, Brianne Lisota1, Jeffery R Jones1, Andrew Petersen1, Anita Bhattacharyya1, Sean P Palecek4, Eric V Shusta4, Christina Kendziorski6, Su-Chun Zhang7,2,8,9.
Abstract
Astrocytes display diverse morphologies in different regions of the central nervous system. Whether astrocyte diversity is attributable to developmental processes and bears functional consequences, especially in humans, is unknown. RNA-seq of human pluripotent stem cell-derived regional astrocytes revealed distinct transcript profiles, suggesting differential functional properties. This was confirmed by differential calcium signaling as well as effects on neurite growth and blood-brain barrier formation. Distinct transcriptional profiles and functional properties of human astrocytes generated from regionally specified neural progenitors under the same conditions strongly implicate the developmental impact on astrocyte diversity. These findings provide a rationale for renewed examination of regional astrocytes and their role in the pathogenesis of psychiatric and neurological disorders.Entities:
Keywords: Astrocyte heterogeneity; Blood-brain barrier; Human astrocyte development; Neuron-glia interaction; RNA-seq
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Year: 2019 PMID： 31189664 PMCID： PMC6633609 DOI： 10.1242/dev.170910
Source DB: PubMed Journal: Development ISSN： 0950-1991 Impact factor: 6.868