Maciej Przybylski1, Tomasz Dzieciątkowski1, Jan Borysowski2, Ryszard Międzybrodzki3,4, Andrzej Górski3,4. 1. Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland. 2. Department of Clinical Immunology, Medical University of Warsaw, Warsaw, Poland, jborysowski@interia.pl. 3. Department of Clinical Immunology, Medical University of Warsaw, Warsaw, Poland. 4. Bacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, Wrocław, Poland.
Abstract
BACKGROUND/AIMS: Bacteriophages (phages) are viruses of bacteria. Escherichia coli phage (T4) can potentially interfere with adsorption of HAdV-5 to cellular integrins by its KGD motif, while staphylococcal A5/80 phage does not possess this structure. The objective of this study was to investigate the effects of T4 and A5/80 phage preparations on type 5 human adenovirus (HAdV-5) DNA synthesis and the expression of HAdV-5 genes. METHODS: Experiments were performed on the A549 cell line. HAdV-5 DNA synthesis was investigated with real-time PCR. Expression of HAdV-5 early (DBP) and late (hexon) genes was determined by quantitative real-time PCR in preincubation and coincubation experiments. RESULTS: While both phage preparations significantly reduced the expression of HAdV-5 genes, synthesis of HAdV-5 DNA was inhibited only by T4. CONCLUSION: Phage preparations show promise as novel antiviral agents. However, further studies are required to investigate their antiviral effects.
BACKGROUND/AIMS: Bacteriophages (phages) are viruses of bacteria. Escherichia coliphage (T4) can potentially interfere with adsorption of HAdV-5 to cellular integrins by its KGD motif, while staphylococcal A5/80 phage does not possess this structure. The objective of this study was to investigate the effects of T4 and A5/80 phage preparations on type 5 human adenovirus (HAdV-5) DNA synthesis and the expression of HAdV-5 genes. METHODS: Experiments were performed on the A549 cell line. HAdV-5 DNA synthesis was investigated with real-time PCR. Expression of HAdV-5 early (DBP) and late (hexon) genes was determined by quantitative real-time PCR in preincubation and coincubation experiments. RESULTS: While both phage preparations significantly reduced the expression of HAdV-5 genes, synthesis of HAdV-5 DNA was inhibited only by T4. CONCLUSION: Phage preparations show promise as novel antiviral agents. However, further studies are required to investigate their antiviral effects.
Authors: Andrzej Górski; Ryszard Międzybrodzki; Ewa Jończyk-Matysiak; Maciej Żaczek; Jan Borysowski Journal: Future Microbiol Date: 2019-09-19 Impact factor: 3.165