Literature DB >> 3118735

Analysis of polyamines as their dabsyl derivatives by reversed-phase high-performance liquid chromatography.

P Koski1, I M Helander, M Sarvas, M Vaara.   

Abstract

The polyamines putrescine, cadaverine, spermidine, and spermine and the corresponding mono-N-acetylpolyamines can be separated as their dimethylaminoazobenzenesulfonyl derivatives in a single analysis in less than 22 min. The method employs reversed-phase high-performance liquid chromatography (Spherisorb S5 ODS2 column) with an acetonitrile/acetate buffer gradient elution system and detection in the visible (436 nm) region. The detection limit for a single dimethylaminoazobenzenesulfonylpolyamine is less than 2 pmol.

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Year:  1987        PMID: 3118735     DOI: 10.1016/0003-2697(87)90395-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  6 in total

1.  Raised plasma polyamine concentrations in patients with severe head injury.

Authors:  J Timothy; C Winter; I Das; N Khan; J De Belleroche
Journal:  J Neurol Neurosurg Psychiatry       Date:  1996-08       Impact factor: 10.154

2.  Polyamines as constituents of the outer membranes of Escherichia coli and Salmonella typhimurium.

Authors:  P Koski; M Vaara
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

3.  Surface-localized spermidine protects the Pseudomonas aeruginosa outer membrane from antibiotic treatment and oxidative stress.

Authors:  Lori Johnson; Heidi Mulcahy; Uliana Kanevets; Yan Shi; Shawn Lewenza
Journal:  J Bacteriol       Date:  2011-12-09       Impact factor: 3.490

4.  Comparison of polyamine and S-adenosylmethionine contents of growing and encysted Acanthamoeba isolates.

Authors:  C M Zhu; A Cumaraswamy; H R Henney
Journal:  Mol Cell Biochem       Date:  1989-10-31       Impact factor: 3.396

5.  Outer Membrane Proteins Derived from Non-cyanobacterial Lineage Cover the Peptidoglycan of Cyanophora paradoxa Cyanelles and Serve as a Cyanelle Diffusion Channel.

Authors:  Seiji Kojima; Koji Muramoto; Tomonobu Kusano
Journal:  J Biol Chem       Date:  2016-08-08       Impact factor: 5.157

6.  The ferrous iron-responsive BqsRS two-component system activates genes that promote cationic stress tolerance.

Authors:  Naomi N Kreamer; Flavia Costa; Dianne K Newman
Journal:  MBio       Date:  2015-02-24       Impact factor: 7.867

  6 in total

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