| Literature DB >> 3118735 |
P Koski1, I M Helander, M Sarvas, M Vaara.
Abstract
The polyamines putrescine, cadaverine, spermidine, and spermine and the corresponding mono-N-acetylpolyamines can be separated as their dimethylaminoazobenzenesulfonyl derivatives in a single analysis in less than 22 min. The method employs reversed-phase high-performance liquid chromatography (Spherisorb S5 ODS2 column) with an acetonitrile/acetate buffer gradient elution system and detection in the visible (436 nm) region. The detection limit for a single dimethylaminoazobenzenesulfonylpolyamine is less than 2 pmol.Entities:
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Year: 1987 PMID: 3118735 DOI: 10.1016/0003-2697(87)90395-2
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365