Sanni Kumar1, Ena Gupta1, Sanket Kaushik1, Vijay Kumar Srivastava1, Juhi Saxena2, Sudhir Mehta3, Anupam Jyoti4. 1. Amity Institute of Biotechnology, Amity University Rajasthan, Amity Education Valley, Kant Kalwar, NH-11C, Jaipur-Delhi Highway, Jaipur 303002, India. 2. Dr. B. Lal Institute of Biotechnology, 6-E, Malviya Industrial Area, Jaipur 302017, India. 3. Department of General Medicine, SMS Medical College & Attached Hospitals, J.L.N. Marg, Jaipur 302004, India. 4. Amity Institute of Biotechnology, Amity University Rajasthan, Amity Education Valley, Kant Kalwar, NH-11C, Jaipur-Delhi Highway, Jaipur 303002, India. Electronic address: ajyoti@jpr.amity.edu.
Abstract
BACKGROUND: Previous study from this lab has discerned oxidative, nitrosative stress and their relationship with cytokines contributing to the severity of sepsis and organ dysfunction. Cytokines are known to induce neutrophil extracellular traps (NETs) formation via free radicals generation. Hyper-activation of neutrophil leads to the increased NETs formation or ineffective clearance of NETs would likely increase the risk of auto-antibody generation against NETs components and being partly responsible for the sepsis severity and organ dysfunction. The present study was undertaken to further assess the status of NETs formation and their correlation with severity of sepsis, with the cytokines and organ dysfunction. METHODS: The level of NETs formation, DNA release, elastase release, and inflammatory cytokines was determined in 80 sepsis patients and 45 healthy volunteers. Their linearity with organ parameters and associations with sepsis severity were also assessed. RESULTS: NETs formation experiment was carried out and it was significantly higher in sepsis (70%) compared to control (30%). NETs % were positively correlated with severity of sepsis and organ dysfunction. Pearson's correlation coefficient demonstrated a direct relation between NETs components and organ parameters with Sepsis severity scores. CONCLUSION: NETs formation is significantly higher due to which it is contributing to the sepsis severity and organ failure.
BACKGROUND: Previous study from this lab has discerned oxidative, nitrosative stress and their relationship with cytokines contributing to the severity of sepsis and organ dysfunction. Cytokines are known to induce neutrophil extracellular traps (NETs) formation via free radicals generation. Hyper-activation of neutrophil leads to the increased NETs formation or ineffective clearance of NETs would likely increase the risk of auto-antibody generation against NETs components and being partly responsible for the sepsis severity and organ dysfunction. The present study was undertaken to further assess the status of NETs formation and their correlation with severity of sepsis, with the cytokines and organ dysfunction. METHODS: The level of NETs formation, DNA release, elastase release, and inflammatory cytokines was determined in 80 sepsispatients and 45 healthy volunteers. Their linearity with organ parameters and associations with sepsis severity were also assessed. RESULTS: NETs formation experiment was carried out and it was significantly higher in sepsis (70%) compared to control (30%). NETs % were positively correlated with severity of sepsis and organ dysfunction. Pearson's correlation coefficient demonstrated a direct relation between NETs components and organ parameters with Sepsis severity scores. CONCLUSION: NETs formation is significantly higher due to which it is contributing to the sepsis severity and organ failure.
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